Repositório Colecção: CDF - FAMO - Book ChaptersCDF - FAMO - Book Chaptershttps://hdl.handle.net/1822/470182024-03-29T07:17:52Z2024-03-29T07:17:52ZLipid-based Nanocarriers for siRNA Delivery: challenges, strategies and the lessons learned from the DODAX: MO Liposomal SystemOliveira, Ana C. N.Fernandes, JoanaGonçalves, AnabelaReal Oliveira, M. Elisabete C.D.Gomes, Andreia Chttps://hdl.handle.net/1822/559312023-10-06T10:56:33Z2018-09-20T15:31:40ZTítulo: Lipid-based Nanocarriers for siRNA Delivery: challenges, strategies and the lessons learned from the DODAX: MO Liposomal System
Autor: Oliveira, Ana C. N.; Fernandes, Joana; Gonçalves, Anabela; Real Oliveira, M. Elisabete C.D.; Gomes, Andreia C
Resumo: The possibility of using the RNA interference (RNAi) mechanisms in gene therapy was one
of the scientific breakthroughs of the last century. Despite the extraordinary therapeutic potential of
this approach, the need for an efficient gene carrier is hampering the translation of the RNAi technology
to the clinical setting. Although a diversity of nanocarriers has been described, liposomes continue
to be one of the most attractive siRNA vehicles due to their relatively low toxicity, facilitated siRNA
complexation, high transfection efficiency and enhanced pharmacokinetic properties.
This review focuses on RNAi as a therapeutic approach, the challenges to its application, namely the
nucleic acids’ delivery process, and current strategies to improve therapeutic efficacy. Additionally,
lipid-based nanocarriers are described, and lessons learned from the relation between biophysical
properties and biological performance of the dioctadecyldimethylammonium:monoolein (DODAX:
MO) system are explored.
Liposomes show great potential as siRNA delivery systems, being safe nanocarriers to protect nucleic
acids in circulation, extend their half-life time, target specific cells and reduce off-target effects. Nevertheless,
several issues related to delivery must be overcome before RNAi therapies reach their full
potential, namely target-cell specificity and endosomal escape. Understanding the relationship between
biophysical properties and biological performance is an essential step in the gene therapy field.
<b>Tipo</b>: article2018-09-20T15:31:40ZLipid-based colloidal carriers for topical application of antiviral drugsLopes, Carla M.Silva, JulianaLúcio, M.Real Oliveira, M. Elisabete C.D.https://hdl.handle.net/1822/512872019-11-06T16:23:54Z2018-03-01T09:31:22ZTítulo: Lipid-based colloidal carriers for topical application of antiviral drugs
Autor: Lopes, Carla M.; Silva, Juliana; Lúcio, M.; Real Oliveira, M. Elisabete C.D.
Resumo: Herpes infections are very frequent infectious diseases, especially in individuals whose immune
system is weakened, causing manifestations in the central nervous system that leave severe
squeals in about 80% of cases. There are several types of etiologic agents of herpes infections,
being the most well-known the Herpes Simplex Virus HSV 1 and 2. HSV 1 usually affects the
face, lips, gums, palate, tongue and nasal mucosa and can spread to other parts of the body and
HSV 2 reaches the genital area, affecting both men and women.
The most common current therapies to treat herpes infections are based on topical
formulations containing an antiviral agent, but whose effectiveness is limited due to low skin
penetration of the active agent, requiring the application of about four or up to five times per
day for improving the therapeutic effectiveness of the formulation. For this reason, the
development of topical formulations containing lipid based colloidal carriers is a promising
approach to promote more effective penetration through the skin and thus to reduce the
frequency of application.
This chapter provides an overview of the current lipid-based colloidal carriers developed for
topical application of antiviral drugs, and summarizes the most important challenges and
strategies that researchers will find when developing formulations with this purpose.
<b>Tipo</b>: bookPart2018-03-01T09:31:22Z6th Iberian Meeting of Colloids and Interfaces - RIC16: book of abstractsReal Oliveira, M. Elisabete C.D.Lucio, MarleneTovar, J.Belsley, M.https://hdl.handle.net/1822/502902018-02-09T13:52:28Z2018-02-09T13:52:28ZTítulo: 6th Iberian Meeting of Colloids and Interfaces - RIC16: book of abstracts
Autor: Real Oliveira, M. Elisabete C.D.; Lucio, Marlene; Tovar, J.; Belsley, M.
<b>Tipo</b>: book2018-02-09T13:52:28ZAdvances towards a miniaturized optical system for gastrointestinal cancer detection using diffuse reflectance and fluorescence spectroscopiesPimenta, SaraCardoso, SusanaCastanheira, Elisabete M. S.Minas, Graçahttps://hdl.handle.net/1822/470232018-02-14T16:31:41ZTítulo: Advances towards a miniaturized optical system for gastrointestinal cancer detection using diffuse reflectance and fluorescence spectroscopies
Autor: Pimenta, Sara; Cardoso, Susana; Castanheira, Elisabete M. S.; Minas, Graça
Resumo: The implementation of a low-cost, miniaturized and minimal invasive microsystem for spectroscopy signals extraction, especially diffuse reflectance and fluorescence, will represent a valuable tool in the detection of gastrointestinal dysplasia. As a progress towards this goal, it is presented the design, optimization, fabrication and characterization (through optical transmittance, selectivity, SEM and profilometry) of 16 MgO/TiO 2 and SiO 2 /TiO 2 based high selective narrow bandpass optical filters to be included in the microsystem. Their performance was successfully evaluated in the extraction of diffuse reflectance and fluorescence signals from tissue phantoms. The obtained results prove their feasibility to correctly extract those spectroscopic signals. Moreover, it was also proved the viability of using a silicon photodiode fabricated in a standard 0.7 µm CMOS process and with an area of 100 × 100 µm2, as photodetector to accurately extract a spectroscopic signal.
<b>Tipo</b>: bookPart