Repositório Colecção: CBFP - Articles
https://hdl.handle.net/1822/69209
CBFP - Articles2024-03-28T17:29:46ZIlluminating Olea europaea L. endophyte fungal community
https://hdl.handle.net/1822/79124
Título: Illuminating Olea europaea L. endophyte fungal community
Autor: Costa, Daniela; Fernandes, Telma; Martins, Fatima; Pereira, Jose A.; Tavares, R. M.; Santos, P. M.; Baptista, Paula; Lino-Neto, T.
Resumo: A wide array of fungal endophytes is known to inhabit plant tissues and were recently recognized as essential for plant health. A better description of the scarcely known endophyte microbiota in olive tree phyllosphere is the first step for elucidating the microbial interactions that lead to olive disease establishment. In this work, the fungal endophytic community of the phyllosphere of different olive tree cultivars (Cobrancosa, Galega vulgar, Madural, Picual, Verdeal Transmontana) is revealed by using a metabarcoding strategy targeting ITS1 barcode. A total of 460 OTUs were obtained, increasing the broad view of fungal endophytes inhabiting the olive tree phyllosphere, in particular yeast endophytes. New endophytes were persistently found in all cultivar tissues. Different olive tree cultivars depicted distinct endophyte communities. Olive cultivars exhibited dissimilar amounts of fungi with distinct ecological functions, which could explain at least in part their differential susceptibility/tolerance to olive diseases.
Descrição: Supplementary material related to this article can be found, in the online version, at doi: https://doi.org/10.1016/j.micres.2020.126693.
<b>Tipo</b>: article2022-08-02T14:12:58ZIn vitro interactions between the ectomycorrhizal Pisolithus tinctorius and the saprotroph Hypholoma fasciculare fungi: morphological aspects and volatile production
https://hdl.handle.net/1822/79122
Título: In vitro interactions between the ectomycorrhizal Pisolithus tinctorius and the saprotroph Hypholoma fasciculare fungi: morphological aspects and volatile production
Autor: Baptista, Paula; de Pinho, Paula Guedes; Moreira, Nathalie; Malheiro, Ricardo; Reis, Francisca; Padrão, Jorge; Tavares, R. M.; Lino-Neto, T.
Resumo: Ectomycorrhizal fungi are crucial for forests sustainability. For Castanea sativa, ectomycorrhizal fungus Pisolithus tinctorius is an important mutualist partner. Saprotrophic fungi Hypholoma fasciculare, although used for biocontrol of Armillaria root disease, it negatively affected the interaction between the P. tinctorius and plant host roots, by compromise the formation of P. tinctorius-C. sativa mycorrhizae. In this work, fungal morphology during inhibition of H. fasciculare against P. tinctorius was elucidated. P. tinctorius growth was strongly affected by H. fasciculare, which was significantly reduced after six days of co-culture and become even more significant through time. During this period, P. tinctorius developed vesicles and calcium oxalate crystals, which were described as mechanisms to stress adaption by fungi. H. fasciculare produced different volatile organic compounds in co-cultures over time and differ between single or in dual-species. H. fasciculare highly produced sesquiterpenes (namely, alpha-muurolene) and nitrogen-containing compounds, which are recognised as having antimicrobial activity.
<b>Tipo</b>: article2022-08-02T14:03:41ZGoing virtual and going wide: comparing Team-Based Learning in-class versus online and across disciplines
https://hdl.handle.net/1822/77031
Título: Going virtual and going wide: comparing Team-Based Learning in-class versus online and across disciplines
Autor: Silva, Elsa Costa e; Lino-Neto, T.; Ribeiro, Eugénia; Rocha, Miguel; Costa, Manuel João
Resumo: Team-based learning (TBL) is an active learning pedagogy developed for in-class sessions and based on the collaborative work of small groups of students. The increasing push to online and blended learning has enhanced the need to expand this pedagogy to a virtual environment, but little evidence has been produced on how students accept online synchronous sessions of TBL. The purpose of this study, that relies on 427 responses, is to present a comparative perspective of traditional in-class versus adapted fully synchronous online TBL and across different disciplinary fields. Students of two different academic years and different programs were surveyed for their acceptance of TBL. They were invited to answer closed-ended questions focused on their engagement in all TBL learning process and the final outcomes provided. Results obtained from this unique comparative study revealed a wide approval of TBL, regardless of the environment (online or in-class TBL sessions), scientific area of courses and student gender. The acceptance of fully online TBL sessions, in a similar way as traditional in-class sessions, could be a rationale for giving more use to the `virtual' context. Other results corroborated previous researches on TBL, such the need of student awareness of TBL benefits to get more engaged in the process or the impact of student activities overload on the TBL process. Implications are informative for pedagogical practice.
<b>Tipo</b>: articleCork oak forests soil bacteria: potential for sustainable agroforest production
https://hdl.handle.net/1822/74786
Título: Cork oak forests soil bacteria: potential for sustainable agroforest production
Autor: Reis, Francisca Rodrigues; Pereira, Ana João; Tavares, R. M.; Baptista, Paula; Lino-Neto, T.
Resumo: Plant growth promoting rhizobacteria (PGPR) are in increasing demand due to their role in promoting sustainable practices, not only in agriculture but also in forestry. Keeping in mind the future application of PGPR for increasing cork oak sustainability, the aim of this study was to find cork oak PGPR isolates with increased nutrient solubilisation traits, able to promote root morphological changes and/or antagonize cork oak bark phytopathogens. Soils from three cork oak forests with distinct bioclimates (humid, semi-humid and semi-arid) were used for isolating bacteria. From the 7634 colony-forming units, 323 bacterial isolates were biochemically assayed for PGPR traits (siderophores production, phosphate solubilizing and organic acids production), and 51 were found to display all these traits. These PGPR were able to induce root morphological changes on <i>Arabidopsis thaliana</i>, like suppression of primary root growth, increase of lateral roots or root hairs formation. However, the most proficient PGPR displayed specific ability in changing a single root morphological trait. This ability was related not only to bacterial genotype, but also with the environment where bacteria thrived and isolation temperature. Bacteria from semi-arid environments (mainly <i>Bacillus megaterium</i> isolates) could hold a promising tool to enhance plant development. Other isolates (<i>Serratia quinivorens</i> or <i>B. cereus</i>) could be further explored for biocontrol purposes.
<b>Tipo</b>: article2021-11-26T10:48:49ZDiversity of fungal endophytic community in Quercus suber L. under different climate scenarios
https://hdl.handle.net/1822/73169
Título: Diversity of fungal endophytic community in Quercus suber L. under different climate scenarios
Autor: Costa, Daniela; Tavares, R. M.; Baptista, Paula; Lino-Neto, T.
Resumo: Cork oak (Quercus suber L.) is an evergreen oak tree species, typically found throughout the Mediterranean Basin, which presents a great ecological and economic importance for Portugal. An increase of cork oak decline due to biotic and abiotic stresses has been reported, which could damage the ecosystem and lower cork production. The attack of some fungal pathogens seems to increase when trees are under several environmental stresses, such as increased temperatures or drought. In this work, fungal endophytic species of cork oak were collected from forest stands in different sites of Portugal. The community of fungal endophytes of young and old twigs was studied by obtaining fungal isolates from plant material and grouping them into morphotypes. This study allowed the comparison of fungal endophytic communities living in cork oak trees from different forests, displaying distinct climates and water availability levels. The results revealed that endophyte communities are better discriminated when considering different plant tissues than cork oak stand location or climate.; O sobreiro (Quercus suber L.) é uma espécie arbórea de folha perene, encontrada tipicamente na Bacia do Mediterrâneo,
que apresenta uma elevada importância ecológica e económica para Portugal. Um aumento no declínio do sobreiro
tem sido associado a situações de stresse biótico e abiótico, o qual resulta em danos no ecossistema e diminuição
da produção de cortiça. A infeção por fungos patogénicos parece aumentar quando os sobreiros se encontram sob
condições ambientais promotoras de stresse, como temperatura elevada e secura. Neste trabalho, as espécies fúngicas
endófitas de sobreiro foram colhidas a partir de exemplares em diferentes locais de Portugal. A comunidade de fungos
endófitos de ramos com diferentes idades foi estudada pela obtenção de isolados a partir deste material vegetal, e pelo
agrupamento dos isolados em morfótipos. Este estudo permitiu a comparação das comunidades fúngicas endófitas em
diferentes povoamentos florestais, sujeitos a condições climáticas distintas e a diferentes níveis de disponibilidade de
água. Os resultados revelam que as comunidades endófitas são distintas entre os diferentes tecidos vegetais, sendo
mais semelhantes entre os locais das florestas ou clima.
<b>Tipo</b>: article2021-05-31T09:49:47Z