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https://hdl.handle.net/1822/10424| Título: | Pulsed electric field reduces the permeability of potato cell wall |
| Autor(es): | Gómez Galindo, Federico Vernier, P. Thomas Dejmek, Petr Vicente, A. A. Gundersen, Martin A. |
| Palavras-chave: | Cell wall FM1-43 eEectric fields Sress Oxidative cross-linking electric fields stress |
| Data: | Mai-2008 |
| Editora: | John Wiley and Sons |
| Revista: | Bioelectromagnetics |
| Citação: | "Bioelectromagnetic". ISSN 0197-8462. 29:4 (May 2008) 296-301. |
| Resumo(s): | The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FM1-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/10424 |
| DOI: | 10.1002/bem.20394 |
| ISSN: | 0197-8462 |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| 2008-024.pdf | 152,75 kB | Adobe PDF | Ver/Abrir |
