Please use this identifier to cite or link to this item:

TitleTranscriptome variability in yeast strains
Author(s)Carreto, Laura
Schuller, Dorit Elisabeth
Santos, Manuel A. S.
KeywordsGenome variability
Transcriptome variability
Saccharomyces cerevisiae
Comparative genome hybridization on array
mRNA profiling
Clinical infections
Natural isolates
Issue dateOct-2008
CitationWORKSHOP ON EVOLUTIONARY AND ENVIRONMENTAL GENOMICS OF YEASTS, Heidelberg, 2008 - “Workshop on Evolutionary and Environmental Genomics of Yeasts”. [S.l. : European Molecular Biology Laboratory, 2008].
Abstract(s)In order to understand yeast natural genome and transcriptome variability and evaluate whether such variability correlates with phenotypic diversity, we have isolated approximately 1000 yeast strains from Portuguese vineyards, namely from the Bairrada and Minho wine regions, genotyped them and selected the most divergent ones for genome and transcriptome characterization, using comparative genome hybridization on array (aCGH) and mRNA profiling, respectively. For comparative purposes, strains isolated from opportunistic clinical infections and commercial strains used by the wine industry were also included in the study. Approximately 3 % of the ORFeome showed copy number alterations relative to the reference strain S288C, which was associated with sub-telomeric instability and Ty element transposition. Interestingly, one third of the Ty elements identified in the genome of the reference laboratory strain (S288C) were absent in the wine strains, but were present in the clinical isolates. Distinct gene functional classes were affected by copy number changes in wine and clinical strains. However, no correlation was found between genome alterations and geographical origin. Transcriptome profiling of the same strains grown in synthetic must, using a common reference design, showed important variation in mRNA levels during fermentation, in particular in the wine isolates. Some genes showed patterns of activation that were consistent with gene copy number differences, while others pointed to strain specific regulation. Substantial variability in expression levels were detected among genes associated with fermentation in the wine strains. Therefore, transcriptome variability is relevant to understand yeast adaptation to new ecological niches, yeast evolution, and also to design strategies to select strains with particular phenotypes.
AccessOpen access
Appears in Collections:DBio - Comunicações/Communications in Congresses

Files in This Item:
File Description SizeFormat 
2008_EMBO_Transcriptome variability_Poster.pdfPoster135,72 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID