Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/13134
Título: | Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers |
Autor(es): | Araújo, Rita Silva, Carla Manuela Pereira Marinho da O'Neill, Jaime Alexandre Antunes Micaelo, N. M. Gübitz, Georg M. Soares, Cláudio M. Casal, Margarida Paulo, Artur Cavaco |
Palavras-chave: | Cutinase Polyester Polyamide 6,6 Site-directed mutagenesis Biocatalysis |
Data: | Mar-2007 |
Editora: | Elsevier |
Revista: | Journal of Biotechnology |
Resumo(s): | Cutinase from Fusarium solani pisi was genetically modified near the active site, by site-directed mutagenesis, to enhance its activity towards polyethylene terephthalate (PET) and polyamide 6,6 (PA 6,6) fibers. The mutations L81A, N84A, L182A, V184A and L189A were done to enlarge the active site in order to better fit a larger polymer chain. Modeling studies have shown enhanced free energy stabilization of model substrate tetrahedral intermediate (TI) bound at the enzyme active site for all mutants, for both model polymers. L81A and L182A showed an activity increase of four- and five-fold, respectively, when compared with the wild type, for PET fibers. L182A showed the one- and two-fold higher ability to biodegrade aliphatic polyamide substrates. Further studies in aliphatic polyesters seem to indicate that cutinase has higher ability to recognize aliphatic substrates. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/13134 |
DOI: | 10.1016/j.jbiotec.2006.12.028 |
ISSN: | 0168-1656 |
Versão da editora: | http://www.sciencedirect.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers.pdf | Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers | 604,4 kB | Adobe PDF | Ver/Abrir |