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TitleMethods to extract the exopolymeric matrix from biofilms : a comparative study
Author(s)Azeredo, Joana
Lazarova, V.
Oliveira, Rosário
Polymeric matrix
Extraction methods
Cell leakage
Issue date1999
PublisherElsevier Science
JournalWater Science and Technology
Citation"Water science & technology". ISSN 0273-1223. 39:7 (1999) 243-250.
Series/Report no.1999;03
Abstract(s)To study the composition of a biofilm a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature; however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that the extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization and the biofilm matrix is solubilized in the solution.
TypeConference paper
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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