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https://hdl.handle.net/1822/14042| Título: | Osteoinduction in human fat derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli |
| Autor(es): | Bessa, Paulo C. Pedro, A. J. Klosch, B. Nobre, Alexandra Griensven, Martijn van Reis, R. L. Casal, Margarida |
| Palavras-chave: | Bone morphogenetic protein-2 Recombinant protein purification Stem cells Tissue engineering |
| Data: | 2008 |
| Editora: | Springer |
| Revista: | Biotechnology Letters |
| Resumo(s): | Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/14042 |
| DOI: | 10.1007/s10529-007-9501-8 |
| ISSN: | 0141-5492 |
| Arbitragem científica: | yes |
| Acesso: | Acesso aberto |
| Aparece nas coleções: | DBio - Artigos/Papers |
