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https://hdl.handle.net/1822/1405
Título: | Effect of hyperbaric stress on yeast morphology: Study by automated image analysis |
Autor(es): | Coelho, M. A. Z. Belo, Isabel Pinheiro, Rita Amaral, A. L. Mota, M. Coutinho, J. A. P. Ferreira, Eugénio C. |
Data: | 2004 |
Editora: | Springer Verlag |
Revista: | Applied Microbiology and Biotechnology |
Citação: | Coelho, M.A.Z., Belo, I., Pinheiro, R. et al. Effect of hyperbaric stress on yeast morphology: study by automated image analysis. Appl Microbiol Biotechnol 66, 318–324 (2004). https://doi.org/10.1007/s00253-004-1648-9 |
Resumo(s): | The effects of hyperbaric stress on the morphology of Saccharomyces cerevisiae were studied in batch cultures under pressures between 0.1 MPa and 0.6 MPa and different gas compositions (air, oxygen, nitrogen or carbon dioxide), covering aerobic and anaerobic conditions. A method using automatic image analysis for classification of S. cerevisiae cells based on their morphology was developed and applied to experimental data. Information on cell size distribution and bud formation throughout the cell cycle is reported. The results show that the effect of pressure on cell activity strongly depends on the nature of the gas used for pressurization. While nitrogen and air to a maximum of 0.6 MPa of pressure were innocuous to yeast, oxygen and carbon dioxide pressure caused cell inactivation, which was confirmed by the reduction of bud cells with time. Moreover, a decrease in the average cell size was found for cells exposed for 7.5 h to 0.6 MPa CO2. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1405 |
DOI: | 10.1007/s00253-004-1648-9 |
ISSN: | 0175-7598 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2004-15.pdf | 166,26 kB | Adobe PDF | Ver/Abrir |