Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/14296| Título: | Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells |
| Autor(es): | Carvalho, P. P. Wu, Xiying Yu, Gang Dietrich, Marilyn Dias, Isabel Gomes, Manuela E. Reis, R. L. Gimble, Jeffrey M. |
| Data: | Mai-2011 |
| Editora: | Informa Healthcare |
| Revista: | Cytotherapy |
| Resumo(s): | Adherent adipose-derived stromal/stem cells (ASC) have been used in pre-clinical regenerative medical studies applied to a broad range of tissues with an ultimate goal of translating these fi ndings to clinical safety and effi cacy testing; however, many protocols passage the cells using porcine-derived trypsin. We have compared porcine trypsin with animal protein-free products from recombinant bacteria (TrypLE Express; Invitrogene) and corn (TrypZean; Sigma) based on cell yield, viability and immunophenotype. ASC harvested with each trypsin product were comparable. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/14296 |
| DOI: | 10.3109/14653249.2010.544721 |
| ISSN: | 1465-3249 1477-2566 |
| Versão da editora: | http://informahealthcare.com/doi/abs/10.3109/14653249.2010.544721 |
| Arbitragem científica: | yes |
| Acesso: | Acesso restrito UMinho |
| Aparece nas coleções: |
Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| e-print Cytotherapy.pdf Acesso restrito! | 187,23 kB | Adobe PDF | Ver/Abrir |
