Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/15152
Título: | Tailoring elastase inhibition with synthetic peptides |
Autor(es): | Vasconcelos, Andreia Azóia, Nuno G. Carvalho, A. C. Gomes, A. C. Guebitz, G. M. Paulo, Artur Cavaco |
Palavras-chave: | Human neutrophil elastase (HNE) Inhibition kinetic Bowman-Birk inhibitor Peptide Wound exudate |
Data: | 2011 |
Editora: | Elsevier |
Revista: | European Journal of Pharmacology |
Resumo(s): | Chronic wounds are the result of excessive amounts of tissue destructive proteases such as human neutrophil elastase (HNE). The high levels of this enzyme found on those types of wounds inactivate the endogenous inhibitor barrier thus, the search for new HNE inhibitors is required. This work presents two new HNE inhibitor peptides, which were synthesized based on the reactive-site loop of the Bowman–Birk inhibitor protein. The results obtained indicated that these new peptides are competitive inhibitors for HNE and, the inhibitory activity can be modulated by modifications introduced at the N- and C-terminal of the peptides. Furthermore, these peptides were also able to inhibit elastase from a human wound exudate while showing no cytotoxicity against human skin fibroblasts in vitro, greatly supporting their potential application in chronic wound treatment. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/15152 |
DOI: | 10.1016/j.ejphar.2011.05.056 |
ISSN: | 0014-2999 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2011 Tailoring elastase inhibition with synthetic peptides.pdf | Tailoring elastase inhibition with synthetic peptides | 735,69 kB | Adobe PDF | Ver/Abrir |