Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/16777

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dc.contributor.authorAzevedo, N. F.-
dc.contributor.authorJardim, T.-
dc.contributor.authorAlmeida, Carina-
dc.contributor.authorCerqueira, L.-
dc.contributor.authorAlmeida, A. J.-
dc.contributor.authorRodrigues, Fernando José dos Santos-
dc.contributor.authorKeevil, C. W.-
dc.contributor.authorVieira, M. J.-
dc.date.accessioned2012-02-03T11:11:40Z-
dc.date.available2012-02-03T11:11:40Z-
dc.date.issued2011-
dc.identifier.issn0003-6072por
dc.identifier.issn1572-9699por
dc.identifier.urihttps://hdl.handle.net/1822/16777-
dc.description.abstractStaphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as Staphylococcus aureus. The method was subsequently successfully adapted for the detection of S. epidermidis in mixed-species blood cultures both by microscopy and flow cytometry.por
dc.description.sponsorshipThis work was supported by the Portuguese Institute Fundacao para a Ciencia e Tecnologia (PhD Fellowship SFRH/BD/29297/2006 and Post-Doc Fellowship SFRH/BPD/42208/2007).por
dc.language.isoengpor
dc.publisherSpringerpor
dc.rightsopenAccesspor
dc.subjectStaphylococcus epidermidispor
dc.subjectBloodstream infectionpor
dc.subjectFlow cytometrypor
dc.subjectFISHpor
dc.subjectNanodiagnosticspor
dc.subjectPermeabilizationpor
dc.titleApplication of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samplespor
dc.typearticlepor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.springerlink.com/por
sdum.publicationstatuspublishedpor
oaire.citationStartPage463por
oaire.citationEndPage470por
oaire.citationIssue3por
oaire.citationTitleAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiologypor
oaire.citationVolume100por
dc.identifier.doi10.1007/s10482-011-9595-9por
dc.identifier.pmid21638111por
dc.subject.wosScience & Technologypor
sdum.journalAntonie van Leeuwenhoekpor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
ICVS - Artigos em revistas internacionais / Papers in international journals

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