Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/16802
Título: | Aqueous two-phase micellar systems in an oscillatory flow micro-reactor: Study of perspectives and experimental performance |
Autor(es): | Lopes, A. M. Silva, Daniel Pereira da Vicente, A. A. Pessoa Júnior, Adalberto Teixeira, J. A. |
Palavras-chave: | screening micro-reactor oscillatory flow mixing aqueous two-phase micellar system triton X-114 affinity ligand glucose-6-phosphate dehydrogenase |
Data: | Set-2011 |
Editora: | Wiley |
Revista: | Journal of Chemical Technology and Biotechnology |
Resumo(s): | Aqueous two-phase micellar systems (ATPMS) are micellar surfactant solutions with physical properties that make them very efficient for the extraction/concentration of biological products. In this work the main proposal that has been discussed is the possible applicability and importance of a novel oscillatory flow micro-reactor (micro-OFR) envisaged for parallel screening and/or development of industrial bioprocesses in ATPMS. Based on the technology of oscillatory flow mixing (OFM), this batch or continuous micro-reactor has been presented as a new small-scale alternative for biological or physical-chemical applications. RESULTS: ATPMS experiments were carried out in different OFM conditions (times, temperatures, oscillation frequencies and amplitudes) for the extraction of glucose-6-phosphate dehydrogenase (G6PD) in Triton X-114/buffer with Cibacron Blue as affinity ligand. CONCLUSION: The results suggest the potential use of OFR, considering this process a promising and new alternative for the purification or pre-concentration of bioproducts. Despite the applied homogenization and extraction conditions have presented no improvements in the partitioning selectivity of the target enzyme, when at rest temperature they have influenced the partitioning behavior in Triton X-114 ATPMS. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/16802 |
DOI: | 10.1002/jctb.2642 |
ISSN: | 1097-4660 |
Outros identificadores: | 0268-2575 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |