Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/17306
Título: | Proteolytic enzyme engineering: a tool for wool |
Autor(es): | Araújo, Rita Silva, Carla Manuela Pereira Marinho da Machado, Raul Casal, Margarida Cunha, A. M. Rodríguez-Cabello, José Carlos Paulo, Artur Cavaco |
Data: | 2009 |
Editora: | ACS Publications |
Revista: | Biomacromolecules |
Citação: | Araújo, R., Silva, C., Machado, R., Casal, M., Cunha, A. M., Rodriguez-Cabello, J. C., & Cavaco-Paulo, A. (2009, May 21). Proteolytic Enzyme Engineering: A Tool for Wool. Biomacromolecules. American Chemical Society (ACS). http://doi.org/10.1021/bm9002943 |
Resumo(s): | One of the goals of protein engineering is to tailor the structure of enzymes to optimize industrial bioprocesses. In the present work, we present the construction of a novel high molecular weight subtilisin, based on the fusion of the DNA sequences coding for Bacillus subtilis prosubtilisin E and for an elastin-like polymer (ELP). The resulting fusion protein was biologically produced in Escherichia coli, purified and used for wool finishing assays. When compared to the commercial protease Esperase, the recombinant subtilisinE-VPAVG220 activity was restricted to the cuticle of wool, allowing a significant reduction of pilling, weight loss and tensile strength loss of wool fibers. Here we report, for the first time, the microbial production of a functionalized high molecular weight protease for controlled enzymatic hydrolysis of wool surface. This original process overcomes the unrestrained diffusion and extended fiber damage which are the major obstacles for the use of proteases for wool finishing applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/17306 |
DOI: | 10.1021/bm9002943 |
ISSN: | 1525-7797 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2009 Proteolytic Enzyme Engineering- A Tool for Wool.pdf | Proteolytic Enzyme Engineering: A Tool for Wool | 1,27 MB | Adobe PDF | Ver/Abrir |