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https://hdl.handle.net/1822/20171| Título: | Comparison of commercial serum-free media for CHO-K1 cell growth and monoclonal antibody production |
| Autor(es): | Rodrigues, E. Costa, A. R. Henriques, Mariana Azeredo, Joana Oliveira, Rosário |
| Palavras-chave: | Serum-free media CHO cells Monoclonal antibody Production Cell growth |
| Data: | 9-Ago-2012 |
| Editora: | Elsevier |
| Revista: | International Journal of Pharmaceutics |
| Resumo(s): | The selection of a serum-free medium for a particular process of production using mammalian cells is a critical step for its success. In this study, seven commercially available serum-free media (EX-CELL, ISF-I, CD CHO, CDM4CHO, CHO-III-A, Octomed and HybridoMed) were evaluated and compared for cell growth and monoclonal antibody (mAb) production of a transfected CHO-K1 cell line. In the conditions assayed, EX-CELL and particularly CDM4CHO are the most recommended media for extended biopharmaceutical processes, on account of inducing superior levels of cell proliferation and mAb production, accentuated by a tendency to improve over time. Furthermore, the less positive results obtained with some media emphasize the importance and the impact of the correct medium selection. |
| Tipo: | Artigo |
| URI: | https://hdl.handle.net/1822/20171 |
| DOI: | 10.1016/j.ijpharm.2012.08.002 |
| ISSN: | 0378-5173 |
| Versão da editora: | http://dx.doi.org/10.1016/j.ijpharm.2012.08.002 |
| Arbitragem científica: | yes |
| Acesso: | Acesso restrito UMinho |
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Ficheiros deste registo:
| Ficheiro | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| 1-s2.0-S037851731200796X-main.pdf Acesso restrito! | Documento principal | 214,78 kB | Adobe PDF | Ver/Abrir |
