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TitlePartial purification and characterisation of Candida nodaensis Killer toxin
Author(s)Silva, Sónia Carina
Aguiar, Cristina
Veríssimo, P.
Pires, E.
Lucas, C.
KeywordsCandida nodaensis
Killer toxin
Partial purification
Preliminary characterisation
Issue date2004
Abstract(s)Preliminary experiments showed that C. nodaensis K factor keeps its biological activity at high NaCl concentrations and is stable after incubation in a relatively broad range of temperature and pH values, whether or not in the presence of salt. The isolation of this K toxin was already achieved by a experimental protocol involving ethanol precipitation of C. nodaensis culture supernatants. Several different approaches are now in progress in order to achieve its purification and further molecular and biochemical characterisation. The identification of the cell wall receptor for this K toxin is also under study, to perform toxin purification by affinity chromatography.
DescriptionComunicação efectuada no Yeast Genetics and Molecular Biology Meeting, Seattle, 2004.
AccessOpen access
Appears in Collections:DBio - Comunicações/Communications in Congresses

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