Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/20448
Título: | Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons |
Autor(es): | Pinto, Luísa Pinheiro, António Mateus Morais, Mónica Bessa, J. M. Sousa, Nuno |
Palavras-chave: | Golgi-cox Immunofluorescence Depression Stress Neuroplasticity Neurogenesis |
Data: | 2012 |
Editora: | Public Library of Science |
Revista: | PLoS ONE |
Resumo(s): | Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple noninvasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/20448 |
DOI: | 10.1371/journal.pone.0033114 |
ISSN: | 1932-6203 |
Versão da editora: | http://www.plosone.org/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Pinto L_PlosOne_2012.pdf | Documento principal | 1,31 MB | Adobe PDF | Ver/Abrir |