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https://hdl.handle.net/1822/20590
Título: | Injectable gellan gum hydrogels with autologous cells for the treatment of rabbit articular cartilage defects |
Autor(es): | Oliveira, J. T. Gardel, Leandro. S. Rada, Tommaso Martins, L. Gomes, Manuela E. Reis, R. L. |
Palavras-chave: | Gellan gum Stem cells Cartilage Tissue engineering In vivo |
Data: | Set-2010 |
Editora: | Wiley |
Revista: | Journal of Orthopaedic Research |
Resumo(s): | In this work, the ability of gellan gum hydrogels coupled with autologous cells to regenerate rabbit full-thickness articular cartilage defects was tested. Five study groups were defined: (a) gellangumwith encapsulated chondrogenic predifferentiated rabbit adipose stem cells (ASCþGF); (b) gellan gum with encapsulated nonchondrogenic predifferentiated rabbit adipose stem cells (ASC); (c) gellan gum with encapsulated rabbit articular chondrocytes (AC) (standard control); (d) gellan gum alone (control); (e) empty defect (control). Fullthickness articular cartilage defects were created and the gellangum constructs were injected and left for 8 weeks. The macroscopic aspect of the explants showed a progressive increase of similarity with the lateral native cartilage, stable integration at the defect site, more pronouncedly in the cell-loaded constructs. Tissue scoring showed that ASCþGF exhibited the best results regarding tissue quality progression. Alcian blue retrieved similar results with a better outcome for the cell-loaded constructs. Regarding real-time PCR analyses, ASCþGF had the best progression with an upregulation of collagen type II and aggrecan, and a downregulation of collagen type I. Gellan gum hydrogels combined with autologous cells constitute a promising approach for the treatment of articular cartilage defects, and adipose derived cellsmayconstitute a valid alternative to currently used articular chondrocytes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/20590 |
DOI: | 10.1002/jor.21114 |
ISSN: | 0736-0266 |
Versão da editora: | http://onlinelibrary.wiley.com/doi/10.1002/jor.21114/pdf |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals ICVS - Artigos em revistas internacionais / Papers in international journals |