Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/22563
Título: | Functionalization of cellulose acetate fibers with engineered cutinases |
Autor(es): | Matamá, Maria Teresa Araújo, Rita Gübitz, Georg M. Casal, Margarida Paulo, Artur Cavaco |
Palavras-chave: | Carbohydrate esterase Functionalization of polymers Cellulose-binding domain Biodegradable |
Data: | 2010 |
Editora: | Wiley |
Revista: | Biotechnology Progress |
Resumo(s): | In the present work, we describe for the first time the specific role of cutinase on surface modification of cellulose acetate fibers. Cutinase exhibits acetyl esterase activity on diacetate and triacetate of 0.010 U and 0.007 U, respectively. An increase on the hydroxyl groups at the fiber surface of 25% for diacetate and 317% for triacetate, after a 24 h treatment, is estimated by an indirect assay. Aiming at further improvement of cutinase affinity toward cellulose acetate, chimeric cutinases are genetically engineered by fusing the 3′-end coding sequence with a bacterial or a fungal carbohydrate-binding module and varying the linker DNA sequence. A comparative analysis of these genetic constructions is presented showing that, the superficial regeneration of cellulose hydrophilicity and reactivity on highly substituted cellulose acetates is achieved by chimeric cutinases. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/22563 |
DOI: | 10.1002/btpr.364 |
ISSN: | 1520-6033 |
Versão da editora: | http://onlinelibrary.wiley.com/journal |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2009 Functionalization of Cellulose Acetate Fibers with Engineered Cutinases.pdf | Functionalization of Cellulose Acetate Fibers with Engineered Cutinases | 825,65 kB | Adobe PDF | Ver/Abrir |