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TitleAntimicrobial pressure of ciprofloxacin and gentamicin on biofilm development by an endoscope-isolated Pseudomonas aeruginosa
Author(s)Machado, Idalina
Graça, Joana
Lopes, H.
Lopes, Susana Patrícia
Pereira, Maria Olívia
KeywordsClinically-relevant biofilms
Issue date2013
PublisherHindawi Limited
JournalISRN Biotechnology
Citationdalina Machado, Joana Graça, Hélder Lopes, Susana Lopes, and Maria O. Pereira, “Antimicrobial Pressure of Ciprofloxacin and Gentamicin on Biofilm Development by an Endoscope-Isolated Pseudomonas aeruginosa,” ISRN Biotechnology, vol. 2013, Article ID 178646, 10 pages, 2013.
Abstract(s)This work aims at characterizing endoscope biofilm-isolated (PAI) and 2 reference strain P. aeruginosa (PA) adhesion, biofilm formation and sensitivity 3 to antibiotics. The recovery ability of the biofilm-growing bacteria subjected to 4 intermittent antibiotic pressure [ciprofloxacin (CIP) and gentamicin (GM)], as 5 well as the development of resistance towards antibiotics and benzalkonium 6 chloride (BC), were also determined. 7 The capacity of both strains to develop biofilms was greatly impaired in the 8 presence of CIP and GM. Sanitization was not complete allowing biofilm 9 recovery after the intermittent cycles of antibiotic pressure. The environmental 10 pressure exerted by CIP and GM did not develop P. aeruginosa resistance to 11 antibiotics nor cross-resistance towards BC. However, data highlighted that 12 none of the antimicrobials led to complete biofilm eradication, allowing the 13 recovery of the remaining adhered population possibly due to the selection of 14 persister cells. This feature may lead to biofilm recalcitrance, reinforce 15 bacterial attachment, and re-colonization of other sites.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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