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TitleDevelopment and characterization of PHB-HV based 3D scaffolds for a tissue engineering and cell-therapy combinatorial approach for spinal cord Injury regeneration
Author(s)Samy, S. M.
Silva, Nuno A.
Correlo, Vitor M.
Fraga, Joana S.
Pinto, Luísa
Teixeira-Castro, Andreia
Leite-Almeida, Hugo
Almeida, Armando
Gimble, Jeffrey M.
Sousa, Nuno
Salgado, António J.
Reis, R. L.
cell-based therapy
PHB-HV 3D scaffolds
spinal cord injury regeneration
tissue engineering
Issue dateAug-2013
JournalMacromolecular Bioscience
CitationRibeiro-Samy S. M., Silva N. A., Correlo V. M., Fraga J. S., Pinto L. A., Teixeira-Castro A., Leite-Almeida H., Almeida A., Gimble J. M., Sousa N., Salgado A. J., Reis R. L. (2013). Development and Characterization of a PHB-HV-based 3D Scaffold for a Tissue Engineering and Cell-therapy Combinatorial Approach for Spinal Cord Injury Regeneration, Macromolecular Bioscience, doi:10.1002/mabi.201300178.
Abstract(s)Spinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)- based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrateco- 3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence, this report addresses the development and characterization of PHB-HV-based 3D scaffolds, produced by freeze-drying, aimed to SCI treatment. The obtained scaffolds reveal an anisotropic morphology with a fully interconnected network of pores. In vitro studies demonstrate a lack of cytotoxic effect of PHB-HV scaffolds. Direct contact assays also reveal their ability to support the culture of CNS-derived cells and mesenchymal-like stem cells from different sources. Finally, histocompatibility studies show that PHB-HV scaffolds are well tolerated by the host tissue, and do not negatively impact the left hindlimb locomotor function recovery. Therefore results herein presented suggest that PHB-HV scaffolds may be suitable for SCI treatment.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals
ICVS - Artigos em revistas internacionais / Papers in international journals

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