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TitleCarbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain
Author(s)Soares, Eduardo V.
Vroman, A.
Mortier, J.
Rijsbrack, K.
Mota, M.
KeywordsCarbon source
NewFlo phenotype
Protein synthesis
Saccharomyces cerevisiae
Issue dateMay-2004
PublisherBlackwell Publishing
JournalJournal of applied microbiology
CitationSoares, E. V., Vroman, A., Mortier, J., Rijsbrack, K., & Mota, M. (2004, May). Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain. Journal of Applied Microbiology. Wiley.
Abstract(s)Aims: To identify the nutrients that can trigger the loss of flocculation under growth conditions in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195. Methods and Results: Flocculation was evaluated using the method of Soares, EX. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]. Yeast growth with metabolizable carbon sources (glucose, fructose, galactose, maltose or sucrose) at 2% (w/v), induced the loss of flocculation in yeast that had previously been allowed to flocculate. The yeast remained flocculent when transferred to a medium containing the required nutrients for yeast growth and a sole nonmetabolizable carbon source (lactose). Transfer of flocculent yeast into a growth medium with ethanol (4% v/v), as the sole carbon source did not induce the loss of flocculation. Even the addition of glucose (2% w/v) or glucose and antimycin A (0.1 mg lˉ¹) to this culture did not bring about loss of flocculation. Cycloheximide addition (15 mglˉ¹) to glucose-growing cells stopped flocculation loss. Conclusions: Carbohydrates were the nutrients responsible for stimulating the loss of flocculation in flocculent yeast cells transferred to growing conditions. The glucose-induced loss of flocculation required de novo protein synthesis. Ethanol prevented glucose-induced loss of flocculation. This protective effect of ethanol was independent of the respiratory function of the yeast. Significance and Impact of the Study: This work contributes to the elucidation of the role of nutrients in the control of the flocculation cycle in NewFlo phenotype yeast strains.
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AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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