Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/2559

TitleCarbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain
Author(s)Soares, Eduardo V.
Vroman, A.
Mortier, J.
Rijsbrack, K.
Mota, M.
KeywordsCarbon source
Flocculation
NewFlo phenotype
Protein synthesis
Saccharomyces cerevisiae
Issue dateMay-2004
PublisherBlackwell Publishing
JournalJournal of applied microbiology
CitationSoares, E. V., Vroman, A., Mortier, J., Rijsbrack, K., & Mota, M. (2004, May). Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain. Journal of Applied Microbiology. Wiley. http://doi.org/10.1111/j.1365-2672.2004.02240.x
Abstract(s)Aims: To identify the nutrients that can trigger the loss of flocculation under growth conditions in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195. Methods and Results: Flocculation was evaluated using the method of Soares, EX. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]. Yeast growth with metabolizable carbon sources (glucose, fructose, galactose, maltose or sucrose) at 2% (w/v), induced the loss of flocculation in yeast that had previously been allowed to flocculate. The yeast remained flocculent when transferred to a medium containing the required nutrients for yeast growth and a sole nonmetabolizable carbon source (lactose). Transfer of flocculent yeast into a growth medium with ethanol (4% v/v), as the sole carbon source did not induce the loss of flocculation. Even the addition of glucose (2% w/v) or glucose and antimycin A (0.1 mg lˉ¹) to this culture did not bring about loss of flocculation. Cycloheximide addition (15 mglˉ¹) to glucose-growing cells stopped flocculation loss. Conclusions: Carbohydrates were the nutrients responsible for stimulating the loss of flocculation in flocculent yeast cells transferred to growing conditions. The glucose-induced loss of flocculation required de novo protein synthesis. Ethanol prevented glucose-induced loss of flocculation. This protective effect of ethanol was independent of the respiratory function of the yeast. Significance and Impact of the Study: This work contributes to the elucidation of the role of nutrients in the control of the flocculation cycle in NewFlo phenotype yeast strains.
TypeArticle
URIhttps://hdl.handle.net/1822/2559
DOI10.1111/j.1365-2672.2004.02240.x
ISSN1364-5072
e-ISSN1365-2672
Publisher versionhttps://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2004.02240.x
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
2004-42-Soares_etal[1].pdf115,29 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID