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https://hdl.handle.net/1822/2559
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Campo DC | Valor | Idioma |
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dc.contributor.author | Soares, Eduardo V. | - |
dc.contributor.author | Vroman, A. | - |
dc.contributor.author | Mortier, J. | - |
dc.contributor.author | Rijsbrack, K. | - |
dc.contributor.author | Mota, M. | - |
dc.date.accessioned | 2005-08-12T14:46:21Z | - |
dc.date.available | 2005-08-12T14:46:21Z | - |
dc.date.issued | 2004-05 | - |
dc.identifier.citation | Soares, E. V., Vroman, A., Mortier, J., Rijsbrack, K., & Mota, M. (2004, May). Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain. Journal of Applied Microbiology. Wiley. http://doi.org/10.1111/j.1365-2672.2004.02240.x | eng |
dc.identifier.issn | 1364-5072 | eng |
dc.identifier.uri | https://hdl.handle.net/1822/2559 | - |
dc.description.abstract | Aims: To identify the nutrients that can trigger the loss of flocculation under growth conditions in an ale-brewing strain, Saccharomyces cerevisiae NCYC 1195. Methods and Results: Flocculation was evaluated using the method of Soares, EX. and Vroman, A. [Journal of Applied Microbiology (2003) 95, 325]. Yeast growth with metabolizable carbon sources (glucose, fructose, galactose, maltose or sucrose) at 2% (w/v), induced the loss of flocculation in yeast that had previously been allowed to flocculate. The yeast remained flocculent when transferred to a medium containing the required nutrients for yeast growth and a sole nonmetabolizable carbon source (lactose). Transfer of flocculent yeast into a growth medium with ethanol (4% v/v), as the sole carbon source did not induce the loss of flocculation. Even the addition of glucose (2% w/v) or glucose and antimycin A (0.1 mg lˉ¹) to this culture did not bring about loss of flocculation. Cycloheximide addition (15 mglˉ¹) to glucose-growing cells stopped flocculation loss. Conclusions: Carbohydrates were the nutrients responsible for stimulating the loss of flocculation in flocculent yeast cells transferred to growing conditions. The glucose-induced loss of flocculation required de novo protein synthesis. Ethanol prevented glucose-induced loss of flocculation. This protective effect of ethanol was independent of the respiratory function of the yeast. Significance and Impact of the Study: This work contributes to the elucidation of the role of nutrients in the control of the flocculation cycle in NewFlo phenotype yeast strains. | eng |
dc.description.sponsorship | This work was supported by Fundo de Apoio à Investigação from Instituto Polite´cnico do Porto (Project P24/96 and P24/97) and by Programa Plurianual de Unidades de I&D CIEA/ISEP. Ann Vroman (KdGH-Antwerpen, Belgium), Jan Mortier and Katleen Rijsbrack (KaHo St Lieven, Belgium) wishes to thank Dr Rob Gille´ and Dr C. Van Keer for the opportunity to participate in the ERASMUS bilateral agreement programme between their schools and ISEP (Portugal). | por |
dc.language.iso | eng | eng |
dc.publisher | Blackwell Publishing | eng |
dc.rights | openAccess | eng |
dc.subject | Carbon source | eng |
dc.subject | Flocculation | eng |
dc.subject | NewFlo phenotype | eng |
dc.subject | Protein synthesis | eng |
dc.subject | Saccharomyces cerevisiae | eng |
dc.title | Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain | eng |
dc.type | article | eng |
dc.peerreviewed | yes | eng |
dc.relation.publisherversion | https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2004.02240.x | - |
sdum.pagination | 1117-1123 | eng |
sdum.publicationstatus | published | eng |
sdum.volume | 96 | eng |
oaire.citationStartPage | 1117 | por |
oaire.citationEndPage | 1123 | por |
oaire.citationIssue | 5 | por |
oaire.citationVolume | 96 | por |
dc.identifier.eissn | 1365-2672 | - |
dc.identifier.doi | 10.1111/j.1365-2672.2004.02240.x | por |
dc.identifier.pmid | 15078529 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of applied microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2004-42-Soares_etal[1].pdf | 115,29 kB | Adobe PDF | Ver/Abrir |