Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/26085
Título: | Encapsulation of RNA molecules in BSA microspheres and internalization into Trypanosoma Brucei parasites and human U2OS cancer cells |
Autor(es): | Shimanovich, Ulyana Tkacz, Itai Dov Eliaz, Dror Paulo, Artur Cavaco Michaeli, S. Gedanken, Aharon |
Palavras-chave: | drug delivery microspheres RNA somochemistry |
Data: | 2011 |
Editora: | Wiley |
Revista: | Advanced Functional Materials |
Resumo(s): | RNA was encapsulated in bovine serum albumin (BSA) microspheres using a one-step sonochemical process from an water–oil solvent biphasic system. Confocal microcoscopy and fluorescence-activated cell sorting indicate that a CY3-RNA (RNA labeled with red fluorescent indocarbocyanine Cy3 dye) sphere is encapsulated in the BSA outer sphere. The diameter of the sphere depends on the number of nucleotides of the RNA, ranging from 0.63 to 2.74 μm. Total RNA (t-RNA) was used as a prototype for the future small interfering RNA (siRNA) delivery. A very broad size distribution characterizes the RNA spheres and therefore, among the loaded BSA spheres, there were sufficiently small spheres to be successfully introduced into trypanosoma brucei parasites and human osteosarcoma U2OS cancer cells. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/26085 |
DOI: | 10.1002/adfm.201100963 |
ISSN: | 1616-3028 |
Versão da editora: | DOI: 10.1002/adfm.201100963 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DET/2C2T - Artigos em revistas internacionais com arbitragem científica |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2011 Encapsulation of RNA Molecules in BSA Microspheres and Internalization into Trypanosoma Brucei Parasites and Human U2OS Cancer Cells.pdf | Encapsulation of RNA Molecules in BSA Microspheres and Internalization into Trypanosoma Brucei Parasites and Human U2OS Cancer Cells | 2,14 MB | Adobe PDF | Ver/Abrir |