Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/2613
Título: | Enzymatic hydrolysis of whey protein concentrates : peptide HPLC profiles |
Autor(es): | Mota, M. V. T. Ferreira, I. M. P. L. V. O. Oliveira, M. B. P. Rocha, Cristina M. R. Teixeira, J. A. Torres, D. Gonçalves, M. P. |
Palavras-chave: | Whey proteins Enzymatic hydrolysis RP-HPLC |
Data: | 2004 |
Editora: | Taylor and Francis |
Revista: | Journal of liquid chromatography & related technologies |
Citação: | "Journal of liquid chromatography & related technologies". ISSN 1082-6076. 27:16 (2004) 2625-2639. |
Resumo(s): | Hydrolysis of whey protein concentrates (WPCs) at different temperatures and pHs, using three enzymes: pepsin, trypsin, and Alcalase®, was monitored during more than 5 hr by reversed phase HPLC/UV, using a column containing a polystyrene-divinylbenzene copolymer-based packing, and an elution gradient from 8% to 80% acetonitrile containing 0.1% TFA. Peptides were separated according to their polarity and size, and degradation of α-lactalbumin (α-la) and β-lactoglobulin (β-lg) was evaluated. The three proteolytic enzymes (pepsin, trypsin, and Alcalase®) employed for hydrolysis of WPCs led to different kinetics of degradation of β-lg. α-la degradation after 15 min was almost complete for the three enzymes. The hydrolysis catalysed by each enzyme resulted in different peptide profiles by HPLC/UV. Hydrolysates produced by pepsin (HP) were resolved into three main fractions of high retention times, while tripsin hydrolysates (HT) were resolved into nine major peaks and Alcalase® hydrolysates (HA) were resolved into 12 major peaks, presenting a wide range of polarities and sizes. Although, with different β-lg hydrolysis extension, chromatographic profiles of the degradation and formation of peptides can be used as a finger print of the type of enzyme used, because peptide profile is not affected either by temperature or pH. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/2613 |
DOI: | 10.1081/JLC-200028429 |
ISSN: | 1082-6076 1520-572X |
Versão da editora: | http://www.taylorandfrancisgroup.com/ http://journalsonline.tandf.co.uk/media/F8GLNVXHWQNGFBG4WR8X/Contributions/6/Q/P/K/6QPKMTKL27XP6A3X.pdf |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
2004-25[2].pdf | 174,89 kB | Adobe PDF | Ver/Abrir |