Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/26324

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dc.contributor.authorAzeredo, Joana-
dc.contributor.authorLazarova, V.-
dc.contributor.authorOliveira, Rosário-
dc.date.accessioned2013-11-27T10:09:27Z-
dc.date.available2013-11-27T10:09:27Z-
dc.date.issued1998-
dc.identifier.urihttps://hdl.handle.net/1822/26324-
dc.description.abstractTo study biofilm composition a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature, however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization. Glutaraldehyde is a bifunctional reagent that binds to cell walls avoiding their permeabilization and the biofilm matrix is solubilized in the solution.por
dc.language.isoengpor
dc.rightsopenAccesspor
dc.subjectBiofilmpor
dc.subjectPolymeric matrixpor
dc.subjectExtraction methodspor
dc.subjectGlutaraldehydepor
dc.subjectCell leakagepor
dc.subjectExopolymerspor
dc.subjectProteinspor
dc.titleMethods to extract the exopolymeric matrix from biofilms: a comparative studypor
dc.typeconferencePaperpor
dc.peerreviewedyespor
sdum.publicationstatuspublishedpor
oaire.citationConferenceDate8 - 10 Oct. 1998por
sdum.event.typeconferencepor
oaire.citationStartPage333por
oaire.citationEndPage340por
oaire.citationConferencePlaceLake Bluff, EUApor
oaire.citationTitleInternational Specialty Conference Microbial Ecology of Biofilms: Concepts, Tools and Applications - Conference Proceedingspor
sdum.conferencePublicationInternational Specialty Conference Microbial Ecology of Biofilms: Concepts, Tools and Applications - Conference Proceedingspor
Appears in Collections:CEB - Artigos em Livros de Atas / Papers in Proceedings

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