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https://hdl.handle.net/1822/26917
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Campo DC | Valor | Idioma |
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dc.contributor.author | Silva, N. A. | - |
dc.contributor.author | Gimble, Jeffrey M. | - |
dc.contributor.author | Sousa, Nuno | - |
dc.contributor.author | Reis, R. L. | - |
dc.contributor.author | Salgado, A. J. | - |
dc.date.accessioned | 2013-12-11T16:00:02Z | - |
dc.date.available | 2013-12-11T16:00:02Z | - |
dc.date.issued | 2013-04 | - |
dc.date.submitted | 2013-11 | - |
dc.identifier.issn | 1547-3287 | por |
dc.identifier.uri | https://hdl.handle.net/1822/26917 | - |
dc.description.abstract | Adipose-derived adult stem cells (ASCs), bone marrow mesenchymal stem cells (bmMSCs), and human umbilical cord perivascular cells (HUCPVCs) tissue have been widely tested for regenerative applications, such as bone regeneration. Moreover, olfactory ensheathing cells (OECs) show promise in promoting spinal cord injury (SCI) regeneration. Our group recently proposed the use of a hybrid scaffold targeting both vertebral bone repair and SCI regeneration. According to this concept, both MSCs and OECs should be in close contact to be influenced by the factors that are involved in secretion. For this reason, here we studied the effects of the OEC secretome on the metabolic activity and proliferation of ASCs, bmMSCs, and HUCPVCs. The stem cells’ secretome effects on metabolic activity and proliferation of the OECs were also considered. In co-cultures of OECs with ASCs, bmMSCs, or HUCPVCs, the metabolic activity/viability, proliferation, and total cell numbers were measured after 2 and 7 days of culture. The results demonstrated that the secretome of OECs has a positive effect on the metabolic activity and proliferation of MSCs from different origins, especially on ASCs. Furthermore, in general, the stem cells’ secretome also had a positive effect on the OECs behavior, particularly when ASCs were in co-culture with OECs. These results suggest that the most suitable combination of cells to be used in our hybrid scaffold is the OECs with the ASCs. Finally, this work adds new knowledge to the cell therapy field, bringing new information about paracrine interactions between OECs and distinct mesenchymal stems | por |
dc.description.sponsorship | The authors would like to acknowledge the Portuguese Foundation for Science and Technology (grant no. PTDC/SAU-BMA/114059/2009; pre-doctoral fellowship to Nuno Silva - SFRH/BD/40684/2007; Ciencia 2007 Program-A.J. Salgado); the Foundation Calouste de Gulbenkian for funds attributed to A.J. Salgado under the scope of the the Gulbenkian Program to Support Research in the Life Sciences; | por |
dc.language.iso | eng | por |
dc.publisher | Mary Ann Liebert Inc. | por |
dc.rights | restrictedAccess | por |
dc.subject | Adipose-derived adult stem cells | por |
dc.subject | Olfactory ensheathing cells | por |
dc.subject | Spinal cord injury | por |
dc.title | Combining adult stem cells and olfactory ensheathing cells: the secretome effect | eng |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://online.liebertpub.com/doi/abs/10.1089/scd.2012.0524 | por |
dc.comments | http://www.3bs.uminho.pt/node/17757 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1232 | por |
oaire.citationEndPage | 1240 | por |
oaire.citationIssue | 8 | por |
oaire.citationTitle | Stem Cells and Development | por |
oaire.citationVolume | 22 | por |
dc.date.updated | 2013-11-27T12:37:04Z | - |
dc.identifier.eissn | 1557-8534 | - |
dc.identifier.doi | 10.1089/scd.2012.0524 | por |
dc.identifier.pmid | 23316915 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Stem Cells and Development | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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17757-NASilva-STEM CELLS AND DEVELOPMENT2013.pdf Acesso restrito! | 427,48 kB | Adobe PDF | Ver/Abrir |