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TitleAdy2p is essential for the acetate permease activity in the yeast Saccharomyces cerevisiae
Author(s)Paiva, Sandra
Devaux, Frederic
Barbosa, Sónia
Jacq, Claude
Casal, Margarida
KeywordsMonocarboxylate transport
Gene expression
Issue dateFeb-2004
PublisherJohn Wiley and Sons
Citation"Yeast". ISSN 1496-8426. 21:3 (2004) 201-210.
Abstract(s)To identify new genes involved in acetate uptake in Saccharomyces cerevisiae, an analysis of the gene expression profiles of cells shifted from glucose to acetic acid was performed. The gene expression reprogramming of yeast adapting to a poor non-fermentable carbon source was observed, including dramatic metabolic changes, global activation of translation machinery, mitochondria biogenesis and the induction of known or putative transporters. Among them, the gene ADY2/YCR010c was identified as a new key element for acetate transport, being homologous to the Yarrowia lipolytica GPR1 gene, which has a role in acetic acid sensitivity. Disruption of ADY2 in S. cerevisiae abolished the active transport of acetate. Microarray analyses of ady2 strains showed that this gene is not a critical regulator of acetate response and that its role is directly connected to acetate transport. Ady2p is predicted to be a membrane protein and is a valuable acetate transporter candidate.
Publisher version
AccessOpen access
Appears in Collections:DBio - Artigos/Papers

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