Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/31922
Título: | Assessing and reducing sources of gene expression variability in Staphylococcus epidermidis biofilms |
Autor(es): | Sousa, Cármen França, Ângela Cerca, Nuno |
Palavras-chave: | biofilms gene expression variability biofilm pool RNA extraction reverse transcriptase qPCR |
Data: | Dez-2014 |
Editora: | Eaton Publishing Company |
Revista: | BioTechniques |
Citação: | Sousa, Cármen; França, Ângela; Cerca, Nuno, Assessing and reducing sources of gene expression variability in S. epidermidis biofilms. BioTechniques, 57(6), 295-301, 2014 |
Resumo(s): | Gene expression quantification can be a useful tool in studying the properties of bacterial biofilms. Unfortunately, techniques such as RNA extraction, cDNA synthesis, and quantitative PCR (qPCR) can introduce variability into mRNA transcript measurements, obscuring biologically relevant results. Here we sought to identify the steps that impair accurate gene expression quantification from Staphylococcus epidermidis biofilm samples. We devised an experimental setup that could be used to determine the contribution of each experimental step to the variability of mRNA transcript measurement. Among factors tested, biofilm growth contributed the most bias to gene expression quantification. Additional experiments demonstrated that pooling biofilms together reduced this variability, resulting in more accurate gene expression analysis results. We therefore recommend pooling in order to reduce the variability associated with gene expression quantification from biofilm samples. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/31922 |
DOI: | 10.2144/000114238 |
ISSN: | 0736-6205 |
Versão da editora: | http://www.biotechniques.com |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18325_1.pdf | 3,8 MB | Adobe PDF | Ver/Abrir |