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https://hdl.handle.net/1822/32110
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Campo DC | Valor | Idioma |
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dc.contributor.author | Machado, Manuela D. | por |
dc.contributor.author | Soares, Eduardo V. | por |
dc.date.accessioned | 2014-12-17T09:57:53Z | - |
dc.date.available | 2014-12-17T09:57:53Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Machado, Manuela D.; Soares, Eduardo V., Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress. Aquatic Toxicology, 147, 1-6, 2014 | por |
dc.identifier.issn | 1879-1514 | por |
dc.identifier.uri | https://hdl.handle.net/1822/32110 | - |
dc.description.abstract | The impact of metals (Cd, Cr, Cu and Zn) on growth, cell volume and cell division of the freshwater alga Pseudokirchneriella subcapitata exposed over a period of 72 h was investigated. The algal cells were exposed to three nominal concentrations of each metal: low (closed to 72 h-EC10 values), intermediate (closed to 72 h-EC50 values) and high (upper than 72 h-EC90 values). The exposure to low metal concentrations resulted in a decrease of cell volume. On the contrary, for the highest metal concentrations an increase of cell volume was observed; this effect was particularly notorious for Cd and less pronounced for Zn. Two behaviours were found when algal cells were exposed to intermediate concentrations of metals: Cu(II) and Cr(VI) induced a reduction of cell volume, while Cd(II) and Zn(II) provoked an opposite effect. The simultaneous nucleus staining and cell image analysis, allowed distinguishing three phases in P. subcapitata cell cycle: growth of mother cell; cell division, which includes two divisions of the nucleus; and, release of four autospores. The exposure of P. subcapitata cells to the highest metal concentrations resulted in the arrest of cell growth before the first nucleus division [for Cr(VI) and Cu(II)] or after the second nucleus division but before the cytokinesis (release of autospores) when exposed to Cd(II). The different impact of metals on algal cell volume and cell-cycle progression, suggests that different toxicity mechanisms underlie the action of different metals studied. The simultaneous nucleus staining and cell image analysis, used in the present work, can be a useful tool in the analysis of the toxicity of the pollutants, in P. subcapitata, and help in the elucidation of their different modes of action. | por |
dc.description.sponsorship | The authors thank the Fundacao para a Ciencia e a Tecnologia (FCT) through the Portuguese Government for their financial support of this work through the strategic project-LA23/2013-2014 to IBB. Manuela D. Machado gratefully acknowledges the post-doctoral grant from FCT (SFRH/BPD/72816/2010). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.rights | openAccess | por |
dc.subject | Algal growth | por |
dc.subject | Biovolume | por |
dc.subject | Cell division | por |
dc.subject | Cell proliferation | por |
dc.subject | Cell size | por |
dc.subject | Metal toxicity | por |
dc.title | Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to heavy metal stress | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | www.elsevier.com/locate/aquatox | - |
dc.comments | CEB17064 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 6 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationTitle | Aquatic Toxicology | por |
oaire.citationVolume | 147 | por |
dc.date.updated | 2014-12-01T11:04:32Z | - |
dc.identifier.eissn | 0166-445X | - |
dc.identifier.doi | 10.1016/j.aquatox.2013.11.017 | por |
dc.identifier.pmid | 24342441 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Aquatic Toxicology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
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document_17064_1.pdf | 879,13 kB | Adobe PDF | Ver/Abrir |