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TitleNaOCl effect on biofilm produced by Staphylococcus aureus isolated from the milking environment and mastitis infected cows
Author(s)Melo, P. C.
Sousa, Cláudia
Botelho, C. M.
Oliveira, Rosário
Nader-Filho, A.
KeywordsStaphylococcus aureus
mastite bovina
hipoclorito de sódio
mastitis infected cows
sodium hypochlorite
Issue date2014
PublisherColégio Brasileiro de Patologia Animal (CBPA). Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
JournalPesquisa Veterinaria Brasileira (brazilian Journal of Veterinary Research)
CitationMelo, P. C.; Sousa, Cláudia; Botelho, C. M.; Oliveira, Rosário; Nader-Filho, A., NaOCl effect on biofilm produced by Staphylococcus aureus isolated from the milking environment and mastitis infected cows. Pesquisa Veterinaria Brasileira (Brazilian Journal of Veterinary Research), 34(2), 109-113, 2014
Abstract(s)Biofilms constitute a physical barrier, protecting the encased bacteria from detergents and sanitizers. The objective of this work was to analyze the effectiveness of sodium hypochlorite (NaOCl) against strains of Staphylococcus aureus isolated from raw milk of cows with subclinical mastitis and Staphylococcus aureus isolated from the milking environment (blowers and milk conducting tubes). The results revealed that, in the presence of NaOCl (150ppm), the number of adhered cells of the twelve S. aureus strains was significantly reduced. When the same strains were evaluated in biofilm condition, different results were obtained. It was found that, after a contact period of five minutes with NaOCl (150ppm), four strains (two strains from milk , one from the blowers and one from a conductive rubber) were still able to grow. Although with the increasing contact time between the bacteria and the NaOCl (150ppm), no growth was detected for any of the strains. Concerning the efficiency of NaOCl on total biofilm biomass formation by each S. aureus strain, a decrease was observed when these strains were in contact with 150 ppm NaOCl for a total period of 10 minutes. This study highlights the importance of a correct sanitation protocol of all the milk processing units which can indeed significantly reduce the presence of microorganisms, leading to a decrease of cow´s mastitis and milk contamination.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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