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TitleSiderophore production by Bacillus megaterium : effect of growth-phase and cultural conditions
Author(s)Santos, S.
Neto, F. F. I.
Machado, Manuela D.
Soares, Helena M. V. M.
Soares, Eduardo V.
KeywordsBacillus megaterium
Carbon source
Environmental-friendly chelating agents
Iron chelator
Siderophore production
Issue date2014
PublisherSpringer Verlag
JournalApplied Biochemistry and Biotechnology
CitationSantos, S.; Neto, F. F. I.; Machado, Manuela D.; Soares, H. M. V. M.; Soares, Eduardo V., Siderophore production by Bacillus megaterium: effect of growth-phase and cultural conditions. Applied Biochemistry and Biotechnology, 172(1), 549-560, 2014
Abstract(s)Siderophore production by Bacillus megaterium was detected, in an iron-deficient culture medium, during the exponential growth phase, prior to the sporulation, in the presence of glucose; these results suggested that the onset of siderophore production did not require glucose depletion and was not related with the sporulation. The siderophore production by B. megaterium was affected by the carbon source used. The growth on glycerol promoted the very high siderophore production (1,182 μmol g−1 dry weight biomass); the opposite effect was observed in the presence of mannose (251 μmol g−1 dry weight biomass). The growth in the presence of fructose, galactose, glucose, lactose, maltose or sucrose, originated similar concentrations of siderophore (546–842 μmol g−1 dry weight biomass). Aeration had a positive effect on the production of siderophore. Incubation of B. megaterium under static conditions delayed and reduced the growth and the production of siderophore, compared with the incubation in stirred conditions.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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