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https://hdl.handle.net/1822/33104
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Campo DC | Valor | Idioma |
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dc.contributor.author | Almeida, Carina | por |
dc.contributor.author | Constante, Diogo | por |
dc.contributor.author | Ferreira, André | por |
dc.contributor.author | Cerqueira, L. | por |
dc.contributor.author | Vieira, M. J. | por |
dc.contributor.author | Azevedo, N. F. | por |
dc.date.accessioned | 2015-01-22T14:44:16Z | - |
dc.date.available | 2015-01-22T14:44:16Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Almeida, Carina; Constante, Diogo; Ferreira, André; Cerqueira, L.; Vieira, M. J.; Azevedo, N. F., A new colorimetric peptide nucleic acid-based assay for the specific detection of bacteria. Future Microbiology, 9(10), 1131-1142, 2014 | por |
dc.identifier.issn | 1746-0913 | por |
dc.identifier.uri | https://hdl.handle.net/1822/33104 | - |
dc.description.abstract | Aim: Developments on synthetic molecules, such as peptide nucleic acid (PNA), make FISH procedures more robust for microbial identification. Fluorochromes use might hinder a broader implementation of PNA-FISH, but colorimetric applications are inexistent so far. Methods: A biotin-labeled eubacteria probe was used to develop a colorimetric PNA-in situ hybridization (ISH) assay. An enzymatic-conjugate, targeting biotin, was introduced. The procedure was optimized and evaluated regarding sensitivity, specificity and detection limit. Results: Results have shown strong ISH signals. The method was specific, but permeabilization problems were observed for Gram-positive bacteria. Detection limit was 5 × 107 CFU/ml, limiting current applications to pre-enriched samples. Conclusion: The PNA-ISH procedure described here is a simple alternative to other detection methods, and is also the base for the development of other PNA colorimetric systems. | por |
dc.language.iso | eng | por |
dc.publisher | Future Medicine | por |
dc.rights | restrictedAccess | por |
dc.subject | Colorimetric detection | por |
dc.subject | ISH | por |
dc.subject | PNA | por |
dc.subject | colorimetric | por |
dc.subject | detection | por |
dc.title | A new colorimetric peptide nucleic acid-based assay for the specific detection of bacteria | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | CEB19335 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1131 | por |
oaire.citationEndPage | 1142 | por |
oaire.citationIssue | 10 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationTitle | Future microbiology | por |
oaire.citationVolume | 9 | por |
dc.date.updated | 2015-01-20T19:50:21Z | - |
dc.identifier.eissn | 1746-0921 | - |
dc.identifier.doi | 10.2217/fmb.14.68 | por |
dc.identifier.pmid | 25405883 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Future microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_19335_1.pdf Acesso restrito! | 3,27 MB | Adobe PDF | Ver/Abrir |