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TitleYeast metabolomics : sample preparation for a GC/MS-based analysis
Author(s)Carneiro, S.
Pereira, R. M.
Rocha, I.
KeywordsMetabolic profiling
Gas chromatography–mass spectrometry
Metabolite extraction
Metabolite derivatization
Issue date26-Mar-2014
JournalMethods in Molecular Biology
CitationCarneiro, S.; Pereira, R. M.; Rocha, I., Yeast metabolomics: sample preparation for a GC/MS-based analysis. In Mapelli, Valeria, ed. lit., "Yeast metabolic engineering : methods and protocols". New York : Springer, 2014. (Methods in molecular biology, vol. 1152). ISBN 978-1-4939-0562-1. p. 197-207.
Abstract(s)Metabolome sample preparation is one of the key factors in metabolomics analyses. The quality of the metabolome data will depend on the suitability of the experimental procedures to the cellular system (e.g., yeast cells) and the analytical performance. Here, we summarize a protocol for metabolome analysis of yeast cells using gas chromatography-mass spectrometry (GC-MS). First, the main phases of a metabolomics analysis are identified: sample preparation, metabolite extraction, and analysis. We also provide an overview on different methods used to quench samples and extract intracellular metabolites from yeast cells. This protocol provides a detailed description of a GC-MS-based analysis of yeast metabolome, in particular for metabolites containing amino and/or carboxyl groups, which represent most of the compounds participating in the central carbon metabolism.
TypeBook part
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:CEB - Livros e Capítulos de Livros / Books and Book Chapters

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