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TitlePartitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate
Author(s)Costa, Angélica Ribeiro da
Coimbra, Jane Sélia dos Reis
Ferreira, Luísa A.
Marcos, João Carlos
Santos, Igor José Boggione
Saldaña, Marleny D. A.
Teixeira, J. A.
Molar mass
Phase diagram
Whey protein
Issue dateJul-2015
PublisherInstitution of Chemical Engineers (IChemE)
JournalFood and Bioproducts Processing
CitationCosta, Angélica Ribeiro da; Coimbra, Jane Sélia dos Reis; Ferreira, Luisa A.; Marcos, João Carlos; Santos, Igor José Boggione; Saldaña, Marleny D. A.; Teixeira, J. A., Partitioning of bovine lactoferrin in aqueous two-phase system containing poly(ethylene glycol) and sodium citrate. Food and Bioproducts Processing, 95, 118-124, 2015
Abstract(s)The partitioning of the whey protein lactoferrin, which is an iron transporter glycoprotein, in an aqueous two-phase system composed of poly(ethylene glycol) (PEG) and sodium citrate was evaluated. Equilibrium data at 25 °C were determined for each system studied using PEG with a molar mass of 1000 and 4000 g·mol1 at pH values of 5.5, 6.5, and 7.5. An increase in the molar mass of the polymer promoted the expansion of the two-phase region and caused the migration of the lactoferrin to the salt-rich bottom phase. An increase in pH also led to the expansion of the biphasic region. However, changing the pH over the tested range slightly affected protein partitioning. Lactoferrin recovery percentages greater than 94% were observed for all of the systems evaluated. The results indicated that lactoferrin can be successfully partitioned in an aqueous two-phase system formed of 14% (w/w) PEG and 10% (w/w) sodium citrate at pH 5.5 and 25 °C. The protein was concentrated 1000-fold in the salt-rich bottom phase in this system.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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