Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/35848
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Castro, Joana | por |
dc.contributor.author | Alves, Patrícia Maria | por |
dc.contributor.author | Sousa, Cármen Sofia Vieira | por |
dc.contributor.author | Cereija, Tatiana | por |
dc.contributor.author | França, Ângela Maria Oliveira Sousa | por |
dc.contributor.author | Jefferson, Kimberly K. | por |
dc.contributor.author | Cerca, Nuno | por |
dc.date.accessioned | 2015-07-01T11:45:27Z | - |
dc.date.available | 2015-07-01T11:45:27Z | - |
dc.date.issued | 2015-06 | - |
dc.date.submitted | 2014-11 | - |
dc.identifier.citation | Castro, Joana; Alves, P.; Sousa, Cármen; Cereija, T.B.; França, A.; Jefferson, K.K.; Cerca, N., Using an in-vitro biofilm model to assess the virulence potential of Bacterial Vaginosis or non-Bacterial Vaginosis Gardnerella vaginalis isolates. Scientific Reports, 5(11640), 2015 | por |
dc.identifier.issn | 2045-2322 | por |
dc.identifier.uri | https://hdl.handle.net/1822/35848 | - |
dc.description.abstract | Gardnerella vaginalis is the most common species found in bacterial vaginosis (BV). However, it is also present in a significant proportion of healthy women and G. vaginalis vaginal colonization does not always lead to BV. In an effort to better understand the differences between G. vaginalis isolated from women with a positive (BV) versus a negative (non-BV) diagnosis of BV, we compared the virulence potential of 7 BV and 7 non-BV G. vaginalis isolates and assessed the virulence factors related to biofilm formation, namely: initial adhesion and cytotoxic effect, biofilm accumulation, susceptibility to antibiotics, and transcript levels of the known vaginolysin, and sialidase genes. Furthermore, we also determined the ability of G. vaginalis to displace lactobacilli previously adhered to HeLa cells. Our results showed that non-BV strains were less virulent than BV strains, as suggested by the lower cytotoxicity and initial adhesion to Hela cells. Significant differences in expression of known virulence genes were also detected, further suggesting a higher virulence potential of the BV associated G. vaginalis. Importantly, we demonstrated that BV associated G. vaginalis were able to displace pre-coated vaginal protective lactobacilli and we hypothesize this to be a trigger for BV development. | por |
dc.description.sponsorship | European Union funds (FEDER/COMPETE) and by national funds (FCT) under the project with reference FCOMP-01-0124-FEDER-008991 (PTDC/BIA-MIC/098228/2008). FCT Strategic Project of UID/BIO/04469/2013 unit the project NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte(ON.2 – O Novo Norte), QREN, FEDER, and the project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). FCT individual fellowship SFRH/BD/93963/2013. | por |
dc.language.iso | eng | por |
dc.publisher | Nature Publishing Group | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/98228/PT | - |
dc.rights | openAccess | por |
dc.subject | Reverse transcription polymerase chain reaction | por |
dc.subject | Biofilms | por |
dc.subject | Infection | por |
dc.subject | Urogenital reproductive disorders | por |
dc.title | Using an in-vitro biofilm model to assess the virulence potential of Bacterial Vaginosis or non-Bacterial Vaginosis Gardnerella vaginalis isolates | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.nature.com/srep/2015/150626/srep11640/full/srep11640.html | por |
dc.comments | CEB22193 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 10 | por |
oaire.citationIssue | 11640 | por |
oaire.citationTitle | Scientific Reports | por |
oaire.citationVolume | 5 | por |
dc.date.updated | 2015-06-30T14:37:27Z | - |
dc.identifier.eissn | 2045-2322 | - |
dc.identifier.doi | 10.1038/srep11640 | por |
dc.identifier.pmid | 26113465 | por |
dc.subject.fos | Ciências Naturais::Ciências Biológicas | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Scientific Reports | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_22193_1.pdf | 1,09 MB | Adobe PDF | Ver/Abrir |