Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/36174

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dc.contributor.authorGonçalves, S.por
dc.contributor.authorPadrão, Jorgepor
dc.contributor.authorRodrigues, I. P.por
dc.contributor.authorSilva, João P.por
dc.contributor.authorSencadas, V.por
dc.contributor.authorLanceros-Méndez, S.por
dc.contributor.authorGirão, H.por
dc.contributor.authorDourado, Fernandopor
dc.contributor.authorRodrigues, L. R.por
dc.date.accessioned2015-07-15T18:03:15Z-
dc.date.available2015-07-15T18:03:15Z-
dc.date.issued2015-
dc.identifier.citationGonçalves, S.; Padrão, Jorge; Rodrigues, I. P.; Silva, João P.; Sencadas, V.; Lanceros-Mendez, S.; Girão, H.; Dourado, Fernando; Rodrigues, L. R., Bacterial cellulose as a support for the growth of retinal pigment epithelium. Biomacromolecules, 16(4), 1341-1351, 2015por
dc.identifier.isbn1526-4602-
dc.identifier.issn1525-7797por
dc.identifier.urihttps://hdl.handle.net/1822/36174-
dc.description.abstractThe feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 m of average thickness) and heatdried BC substrates were surface modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography and also regarding their permeability, dimensional stability, mechanical properties and endotoxin content. Then, their ability to promote RPE cell adhesion and proliferation in vitro was assessed. All surface-modified BC substrates presented similar permeation coefficients with solutes of up to 300 kDa. Acetylation of BC decreased its swelling and the amount of endotoxins. Surface modification of BC greatly enhanced the adhesion and proliferation of RPE cells. All samples showed similar stress-strain behavior; BC and acetylated BC showed the highest elastic modulus, but the latter exhibited a slightly smaller tensile strength and elongation at break as compared to pristine BC. Although similar proliferation rates were observed among the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation.por
dc.description.sponsorshipThe authors acknowledge the Portuguese Foundation for Science and Technology (FCT, Portugal) for the financial support provided by the Research Grants SERH/BD/63578/2009, SFRH/BD/64901/2009, SFRH/BPD/64958/2009, and SFRH/BPD/63148/2009 for S.G., J.P., J.P.S., and V.S., respectively. The authors also acknowledge the Projects PEst-OE/EQB/LA0004/2013, PEst-OE/EQB/LA0023/2013, PTDC/BBB-BQB/2450/2012, and RECI/BBB-EBI/0179/2012 (Number: FCOMP-01-0124-FEDER-027462), cofunded by QREN, FEDER.por
dc.language.isoengpor
dc.publisherACS Publicationspor
dc.relation.hasversionhttp://hdl.handle.net/1822/41789-
dc.rightsopenAccesspor
dc.titleBacterial cellulose as a support for the growth of retinal pigment epitheliumpor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://pubs.acs.org/journal/bomaf6por
dc.commentsCEB19851por
sdum.publicationstatuspublishedpor
oaire.citationStartPage1341por
oaire.citationEndPage1351por
oaire.citationIssue4por
oaire.citationTitleBiomacromoleculespor
oaire.citationVolume16por
dc.date.updated2015-05-06T10:46:20Z-
dc.identifier.eissn1526-4602-
dc.identifier.doi10.1021/acs.biomac.5b00129por
dc.identifier.pmid25748276por
dc.subject.wosScience & Technologypor
sdum.journalBiomacromoleculespor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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