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TitleHierarchical scaffolds enhance osteogenic differentiation of human Wharton’s jelly derived stem cells
Author(s)Canha-Gouveia, A.
Costa-Pinto, A. R.
Martins, Albino
Silva, N. A.
Faria, Susana
Sousa, R. A.
Salgado, A. J.
Sousa, Nuno
Reis, R. L.
Neves, N. M.
Hierarchical fibrous scaffolds
Osteogenic differentiation
Rapid prototyping
Issue dateSep-2015
PublisherIOP Publishing
CitationCanha-Gouveia A., Costa-Pinto A. R., Martins A., Silva N. A., Faria S., Sousa R. A., Salgado A. J., Sousa N., Reis R. L., Neves N. M. Hierarchical scaffolds enhance osteogenic differentiation of human Wharton’s Jelly derived Stem Cells, Biofabrication, Vol. 7, Issue 3, pp. 035009, doi:10.1088/1758-5090/7/3/035009, 2015
Abstract(s)Hierarchical structures, constituted by polymeric nano and microfibers, have been considered promising scaffolds for tissue engineering strategies, mainly because they mimic, in some way, the complexity and nanoscale detail observed in real organs. The chondrogenic potential of these scaffolds was already demonstrated, but their osteogenic potential is not corroborated yet. In order to assess if a hierarchical structure, with nanoscale details incorporated, is an improved scaffold for bone tissue regeneration, we evaluate cell adhesion, proliferation, and osteogenic differentiation of human Whartonâ s jelly derived stem cells (hWJSCs), seeded into hierarchical fibrous scaffolds. Biological data corroborates that hierarchical fibrous scaffolds show an enhanced cell entrapment when compared to rapid prototyped scaffolds without nanofibers. Furthermore, upregulation of bone specific genes and calcium phosphate deposition confirms the successful osteogenic differentiation of hWJSCs on these scaffolds. These results support our hypothesis that a scaffold with hierarchical structure, in conjugation with hWJSCs, represents a possible feasible strategy for bone tissue engineering applications.
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AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals
ICVS - Artigos em revistas internacionais / Papers in international journals

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