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TitleAssessment of liposome disruption to quantify drug delivery in vitro
Author(s)Nogueira, E.
Cruz, Célia F.
Loureiro, A.
Nogueira, Patrícia
Freitas, Jaime
Moreira, Alexandra
Carmo, Alexandre M.
Gomes, Andreia C.
Preto, Ana
Cavaco-Paulo, Artur
KeywordsDrug delivery
Hoechst 34,580
in vitro methodology
Liposome disruption
Issue dateFeb-2016
PublisherElsevier B.V.
JournalBiochimica et Biophysica Acta (BBA). Biomembranes
CitationNogueira, E.; Cruz, Celia F; Loureiro, A.; Nogueira, Patrícia; Freitas, Jaime; Moreira, Alexandra; Carmo, Alexandre M.; Gomes, Andreia C.; Preto, Ana; Paulo, Artur Cavaco, Assessment of liposome disruption to quantify drug delivery in vitro. Biochimica et Biophysica Acta - Biomembranes, 1858(2), 163-167, 2016
Abstract(s)Efficient liposome disruption inside the cells is a key for success with any type of drug delivery system. The efficacy of drug delivery is currently evaluated by direct visualization of labeled liposomes internalized by cells, not addressing objectively the release and distribution of the drug. Here, we propose a novel method to easily assess liposome disruption and drug release into the cytoplasm. We propose the encapsulation of the cationic dye Hoechst 34,580 to detect an increase in blue fluorescence due to its specific binding to negatively charged DNA. For that, the dye needs to be released inside the cell and translocated to the nucleus. The present approach correlates the intensity of detected fluorescent dye with liposome disruption and consequently assesses drug delivery within the cells.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
DBio - Artigos/Papers

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