Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/40198
Título: | Exploring female mice interstrain differences relevant for models of depression |
Autor(es): | Calçada, Daniela Alexandra Sá Roque, Susana Branco, Carlos Monteiro, Susana Rodrigues, Bruno Cerqueira Sousa, Nuno Palha, Joana Almeida Neves, Margarida Correia |
Palavras-chave: | Mouse strains Depressive-like behavior Anxious-like behavior Cytokines Dentate gyrus neurons' morphology Hippocampal cell proliferation Females |
Data: | Dez-2015 |
Editora: | Frontiers Media |
Revista: | Frontiers in Behaviroral Nerociences |
Resumo(s): | Depression is an extremely heterogeneous disorder. Diverse molecular mechanisms have been suggested to underlie its etiology. To understand the molecular mechanisms responsible for this complex disorder, researchers have been using animal models extensively, namely mice from various genetic backgrounds and harboring distinct genetic modifications. The use of numerous mouse models has contributed to enrich our knowledge on depression. However, accumulating data also revealed that the intrinsic characteristics of each mouse strain might influence the experimental outcomes, which may justify some conflicting evidence reported in the literature. To further understand the impact of the genetic background, we performed a multimodal comparative study encompassing the most relevant parameters commonly addressed in depression, in three of the most widely used mouse strains: Balb/c, C57BL/6, and CD-1. Moreover, female mice were selected for this study taken into account the higher prevalence of depression in women and the fewer animal studies using this gender. Our results show that Balb/c mice have a more pronounced anxious-like behavior than CD-1 and C57BL/6 mice, whereas C57BL/6 animals present the strongest depressive-like trait. Furthermore, C57BL/6 mice display the highest rate of proliferating cells and brain-derived neurotrophic factor (Bdnf) expression levels in the hippocampus, while hippocampal dentate granular neurons of Balb/c mice show smaller dendritic lengths and fewer ramifications. Of notice, the expression levels of inducible nitric oxide synthase (iNos) predict 39.5% of the depressive-like behavior index, which suggests a key role of hippocampal iNOS in depression. Overall, this study reveals important interstrain differences in several behavioral dimensions and molecular and cellular parameters that should be considered when preparing and analyzing experiments addressing depression using mouse models. It further contributes to the literature by revealing the predictive value of hippocampal iNos expression levels in depressive-like behavior, irrespectively of the mouse strain. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/40198 |
DOI: | 10.3389/fnbeh.2015.00335 |
ISSN: | 1662-5153 |
Versão da editora: | http://journal.frontiersin.org/journal/behavioral-neuroscience |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
fnbeh-09-00335.pdf | 1,93 MB | Adobe PDF | Ver/Abrir |