Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/41
Título: | Automated image analysis to improve bead ingestion toxicity test counts in the protozoan Tetrahymena pyriformis |
Autor(es): | Dias, Nicolina Amaral, A. L. Ferreira, Eugénio C. Lima, Nelson |
Palavras-chave: | Tetrahymena pyriformis Image analysis Ingestion Toxicity tests Bead counts |
Data: | 2003 |
Editora: | Wiley |
Revista: | Letters in Applied Microbiology |
Citação: | Dias, N., Amaral, A.L., Ferreira, E.C. and Lima, N. (2003), Automated image analysis to improve bead ingestion toxicity test counts in the protozoan Tetrahymena pyriformis. Letters in Applied Microbiology, 37: 230–233. doi:10.1046/j.1472-765X.2003.01382.x |
Resumo(s): | Aims: To improve bead ingestion counts in Tetrahymena pyriformis by automated image analysis as an alternative to direct-counts. Methods and Results: Fluorescent latex beads were added to T. pyriformis cultures for ingestion tests. The number of beads ingested by 25 cells was counted directly by epifluorescence microscopy and compared with similar data from image analysis. ANOVA indicated that counts were not significantly different (P < 0.05). The image analysis particularly provided advantages in terms of speed. Conclusions: The image analysis is superior to direct beads counting in T. pyriformis particularly in terms of speed of analysis. Significance and Impact of the Study: The image analysis method is very rapid and will allow many more toxicological analyses to be undertaken with less operator error. |
Tipo: | Artigo |
Descrição: | Prova de tipográfica (In Press). |
URI: | https://hdl.handle.net/1822/41 |
DOI: | 10.1046/j.1472-765X.2003.01382.x |
ISSN: | 0266-8254 |
e-ISSN: | 1472-765X |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DCILM - Artigos (Papers) |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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lam1382.pdf | 102,03 kB | Adobe PDF | Ver/Abrir |