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TitleLaccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties
Author(s)Sampaio, Liliana M. P.
Padrão, Jorge
Faria, Jorge
Silva, João P.
Silva, Carla J.
Dourado, Fernando
Zille, Andrea
KeywordsBacterial Cellulose
Wound Dressing
Issue date10-Jul-2016
JournalCarbohydrate Polymers
CitationSampaio, Liliana M. P.; Padrão, Jorge; Faria, Jorge; Silva, João P.; Silva, Carla J.; Dourado, Fernando; Zille, Andrea, Laccase immobilization on bacterial nanocellulose membranes: antimicrobial, kinetic and stability properties. Carbohydrate Polymers, 145, 1-12, 2016
Abstract(s)This work studied the physical immobilization of a commercial laccase on bacterial nanocellulose (BNC) aiming to identify the laccase antibacterial properties suitable for wound dressings. Physico-chemical analysis demonstrates that the BNC structure is manly formed by pure crystalline I cellulose. The pH optimum and activation energy of free laccase depends on the substrate employed corresponding to pH 6, 7, 3 and 57, 22, 48 kJ mol1 for 2,6-dimethylphenol (DMP), catechol and 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), respectively. The Michaelis-Menten constant (Km) value for the immobilized laccase (0.77 mM) was found to be almost double of that of the free enzyme (0.42 mM). However, the specific activities of immobilized and free laccase are similar suggesting that the cage-like structure of BNC allows entrapped laccase to maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase in Gram-positive (92%) and Gram-negative (26%) bacteria and cytotoxicity acceptable for wound dressing applications.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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