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TitleCa2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor
Author(s)Pandey, Kishor
Ferreira, Pedro Eduardo Mendes
Ishikawa, Takeshi
Nagai, Takeharu
Kaneko, Osamu
Yahata, Kazuhide
Issue dateMar-2016
PublisherNature Publishing Group
JournalScientific Reports
CitationPandey, K., Ferreira, P. E., Ishikawa, T., Nagai, T., Kaneko, O., & Yahata, K. (2016). Ca2+ monitoring in Plasmodium falciparum using the yellow cameleon-Nano biosensor. Scientific reports, 6. doi: 10.1038/srep23454
Abstract(s)Calcium (Ca2+)-mediated signaling is a conserved mechanism in eukaryotes, including the human malaria parasite, Plasmodium falciparum. Due to its small size (<10?µm) measurement of intracellular Ca2+ in Plasmodium is technically challenging, and thus Ca2+ regulation in this human pathogen is not well understood. Here we analyze Ca2+ homeostasis via a new approach using transgenic P. falciparum expressing the Ca2+ sensor yellow cameleon (YC)-Nano. We found that cytosolic Ca2+ concentration is maintained at low levels only during the intraerythrocytic trophozoite stage (30?nM), and is increased in the other blood stages (>300?nM). We determined that the mammalian SERCA inhibitor thapsigargin and antimalarial dihydroartemisinin did not perturb SERCA activity. The change of the cytosolic Ca2+ level in P. falciparum was additionally detectable by flow cytometry. Thus, we propose that the developed YC-Nano-based system is useful to study Ca2+ signaling in P. falciparum and is applicable for drug screening.
Publisher version
AccessOpen access
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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