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TitleAntimicrobial enzyme immobilization in bacterial cellulose
Author(s)Sampaio, Liliana M. P.
Ribeiro, Jorge Manuel Padrão
Faria, Jorge
Silva, João Pedro Martins Soares Castro
Silva, Carla J.
Dourado, Fernando
Zille, Andrea
KeywordsBacterial nanocellulose
Issue date2017
Abstract(s)This work studied the physical immobilization of laccase on bacterial nanocellulose (BNC) aiming to identify the enzyme antibacterial properties suitable for wound dressings. The pH optimum and activation energy of free laccase depends on the substrate employed. The Michaelis-Menten constant for the immobilized laccase was found to be almost double of that of the free enzyme. However, the specific activities of immobilized and free laccase are similar suggesting that entrapped laccase on BNC maintain some flexibility and favour substrate accessibility. The results clearly show the antimicrobial effect of laccase and cytotoxicity acceptable for wound dressing applications.
TypeOral presentation
AccessOpen access
Appears in Collections:DET/2C2T - Comunicações em congressos internacionais com arbitragem científica

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