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https://hdl.handle.net/1822/48381
Título: | Neospora caninum: high susceptibility to the parasite in C57BL/10ScCr mice |
Autor(es): | Botelho, A. S. Teixeira, L. Costa, J. M. Correia da Faustino, A. M. R. Castro, António G. Vilanova, M. |
Palavras-chave: | Neospora caninum acute infection Apicomplexa C57BL/10 ScCr (ScCr) mice C57BL/10 ScSn (ScSn) mice NcT Neospora caninum tachyzoites IFN-γ, interferon-γ IL-4, interleukin-4 IL-12, interleukin-12 TLR4, Toll-like receptor 4 IL-12R, IL-12 receptor Th1, T helper 1 PBS, phosphate-buVered saline RNA, ribonucleic acid DNA, deoxyribonucleic acid mRNA, messenger ribonucleic acid cDNA, RNA to complementary DNA PCR, polymerase chain reaction HPRT, hypoxanthine phosphoribosyltransferase dNTPs, deoxynucleotide triphosphate mix interferon-gamma interleukin-4 interleukin-12 toll-like receptor 4 IL-12 receptor T helper 1 phosphate-buffered saline ribonucleic acid deoxyribonucleic acid messenger ribonucleic acid polymerase chain reaction hypoxanthine phosphoribosyltransferase deoxynucleotide triphosphate mix cDNA DNA dNTPs HPRT IFN-? IL-12 IL-12R IL-4 interferon-? mRNA PBS PCR RNA RNA to complementary DNA Th1 TLR4 IFN-γ interferon-γ |
Data: | 2007 |
Editora: | Elsevier |
Revista: | Experimental Parasitology |
Resumo(s): | C57BL/10ScCr mice, lack Toll-like receptor 4 and a functional Interleukin-12 receptor. Taking this into account, susceptibility of these mice to Neospora caninum infection was assessed comparatively to that of immunocompetent C57BL/10ScSn mice. C57BL/10ScCr mice inoculated intraperitoneally with 5 x 10(5) N. caninum tachyzoites showed a high Susceptibility to this parasite. All infected C57BL/10ScCr mice were dead by day 8 post-infection whereas all control C57BL/10ScSn mice survived this parasitic challenge. Immunohistochemical analysis of infected C57BL/10ScCr mice showed N. caninum tachyzoites spread in the pancreas, liver, lung, intestine, heart and brain whereas no parasites were detected in similarly infected C57BL/10ScSn controls. The higher susceptibility of C57BL/10ScCr mice to neosporosis correlates with reduced interferon-gamma mRNA expression and increased IL-4 mRNA expression, comparatively to C57BL/10ScSn controls, detected in the spleen after the parasitic challenge. C57BL/10ScCr mice could thus be used as a new experimental model where to study immunobiological mechanisms associated with host Susceptibility to neosporosis. |
Tipo: | Artigo |
Descrição: | Neospora caninum: high susceptibility to the parasite in C57BL/10ScCr mice |
URI: | https://hdl.handle.net/1822/48381 |
DOI: | 10.1016/j.exppara.2006.06.004 |
ISSN: | 0014-4894 |
e-ISSN: | 1090-244 |
Versão da editora: | http://www.sciencedirect.com/science/article/pii/S0014489406001457 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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botelho as_experimental parasitology 2007.pdf | 1,18 MB | Adobe PDF | Ver/Abrir |