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TitleMolecular characterization of short-term primary cultures and comparison with corresponding tumor tissue of Brazilian glioblastoma patients
Author(s)Cruvinel-Carloni, Adriana
Silva-Oliveira, Renato
Torrieri, Raul
Bidinotto, Lucas Tadeu
Berardinelli, Gustavo Noriz
Oliveira-Silva, Viviane Aline
Clara, Carlos Afonso
Almeida, Gisele Caravina de
Martinho, Olga
Squire, Jeremy Andrew
Reis, R. M.
KeywordsChromosomal aberrations
Primary culture
Suppressor genes
Issue date13-Apr-2017
PublisherAME Publishing Company
JournalTranslational Cancer Research
CitationCruvinel-Carloni, A., SilvaOliveira, R., Torrieri, R., Bidinotto, L. T., Berardinelli, G. N., Oliveira-Silva, V. A., ... & Reis, R. M. (2017). Molecular characterization of short-term primary cultures and comparison with corresponding tumor tissue of Brazilian glioblastoma patients. Translational Cancer Research, 6(2), 332-345
Abstract(s)Background: Glioblastoma, the most frequent and malignant adult brain tumor, has been extensively studied. However, there is no effective treatment, and to overcome this challenging scenario, it is essential to improve preclinical biological models. This study aimed to molecularly characterize short-term glioblastoma primary cultures and to compare them with patient tumor profiles. Methods: Glioblastoma cell lines were established from Barretos Cancer Hospital patients diagnosed with glioblastoma. The cells were cultured with DMEM (+)10% FBS (+)1% PS and were molecularly characterized using array CGH (aCGH), next-generation and Sanger sequencing. Results: We established four short-term glioblastoma cultures and we found that the primary cells exhibited a diversity of chromosomal aberrations, with gain of chromosome 7 and loss of chromosomes 10, 13 and 17p being the most frequent alterations. Mutation profiling showed that hotspot TERT promoter mutations were present in 3/4 cases, followed by mutations in TP53 (2/4) and in the RB1, BRAF and PTEN (1/4) genes. A similar chromosomal and mutation pattern was observed in all short-term cultures and matched frozen tumors. Conclusions: Herein, short-term glioblastoma primary cultures were successfully characterized and had genetic make-ups that were similar to those of patient tumors, suggesting that short-term primary cultures are suitable in vitro models for studies of glioblastoma biology.
Publisher version
AccessOpen access
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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