Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/49771
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Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Miranda-Gonçalves, Vera | por |
dc.contributor.author | Carneiro, Diana | por |
dc.contributor.author | Valbom, Inês | por |
dc.contributor.author | Cury, Fernanda Paula | por |
dc.contributor.author | Silva, Viviane Aline | por |
dc.contributor.author | Granja, Sara Costa | por |
dc.contributor.author | Reis, R. M. | por |
dc.contributor.author | Baltazar, Fátima | por |
dc.contributor.author | Martinho, Olga | por |
dc.date.accessioned | 2018-01-26T16:48:27Z | - |
dc.date.available | 2018-01-26T16:48:27Z | - |
dc.date.issued | 2017-11-27 | - |
dc.identifier.citation | Miranda-Gonçalves, V., Cardoso-Carneiro, D., Valbom, I., Cury, F. P., Silva, V. A., Granja, S., ... & Martinho, O. (2017). Metabolic alterations underlying Bevacizumab therapy in glioblastoma cells. Oncotarget, 8(61), 103657. | por |
dc.identifier.issn | 1949-2553 | - |
dc.identifier.uri | https://hdl.handle.net/1822/49771 | - |
dc.description.abstract | Anti-VEGF therapy with Bevacizumab is approved for glioblastoma treatment, however, it is known that tumors acquired resistance and eventually became even more aggressive and infiltrative after treatment. In the present study we aimed to unravel the potential cellular mechanisms of resistance to Bevacizumab in glioblastoma in vitro models. Using a panel of glioblastoma cell lines we found that Bevacizumab is able to block the secreted VEGF by the tumor cells and be internalized to the cytoplasm, inducing cytotoxicity in vitro. We further found that Bevacizumab increases the expression of hypoxic (HIF-1α and CAIX) and glycolytic markers (GLUT1 and MCT1), leading to higher glucose uptake and lactate production. Furthermore, we showed that part of the consumed glucose by the tumor cells can be stored as glycogen, hampering cell dead following Bevacizumab treatment. Importantly, we found that this change on the glycolytic metabolism occurs independently of hypoxia and before mitochondrial impairment or autophagy induction. Finally, the combination of Bevacizumab with glucose uptake inhibitors decreased in vivo tumor growth and angiogenesis and shift the expression of glycolytic proteins. In conclusion, we reported that Bevacizumab is able to increase the glucose metabolism on cancer cells by abrogating autocrine VEGF in vitro. Define the effects of anti-angiogenic drugs at the cellular level can allow us to discover ways to revert acquired resistance to this therapeutic approaches in the future | por |
dc.description.sponsorship | This study was partially developed under the scope of the project NORTE-01-0145-FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), and through the Competitiveness Factors Operational Programme (COMPETE), by Portuguese funds, through the Foundation for Science and Technology (FCT), under the scope of the project POCI-01-0145-FEDER-007038, and by Brazilian MCTI/CNPq No 73/2013. VMG was recipient from a PhD fellowship (SFRH/BD/51997/2012) from Fundação para a Ciência e Tecnologia (FCT), Portugal. FC was recipient of a master FAPESP fellowship (n° 2014/03684-0) and “BEPE - Bolsa Estágio de Pesquisa no Exterior” (n° 2015/02691-6). OM is recipient of a post-doc fellowship (SFRH/BPD/108351/2015) from FCT, Portugal | por |
dc.language.iso | eng | por |
dc.publisher | Impact Journals | por |
dc.rights | openAccess | por |
dc.subject | Anti-angiogenic therapy | por |
dc.subject | Bevacizumab | por |
dc.subject | Glioblastoma | por |
dc.subject | Glycolytic metabolism | por |
dc.title | Metabolic alterations underlying Bevacizumab therapy in glioblastoma cells | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=view&path[]=21761&path[]=69048 | por |
oaire.citationStartPage | 103657 | por |
oaire.citationEndPage | 103670 | por |
oaire.citationIssue | 61 | por |
oaire.citationVolume | 8 | por |
dc.date.updated | 2018-01-09T10:02:29Z | - |
dc.identifier.doi | 10.18632/oncotarget.21761 | por |
dc.subject.fos | Ciências Médicas::Medicina Básica | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Oncotarget | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |