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https://hdl.handle.net/1822/50152
Título: | Brewer’s spent grains protects against oxidative DNA damage in Saccharomyces cerevisiae |
Autor(es): | Moreira, Manuela M. Carvalho, Daniel O. Oliveira, Rui Pedro Soares de Johansson, Björn Guido, Luís F. |
Palavras-chave: | Barley Brewer's spent grain Phenolic compounds Antigenotoxic activity Yeast comet assay Brewer's yeast |
Data: | 15-Jun-2017 |
Editora: | Canadian Center of Science and Education (CCSE) |
Revista: | Journal of Agricultural Science |
Citação: | Manuela M. Moreira, Daniel O. Carvalho, Rui Oliveira, Björn Johansson & Luís F. Guido. 2017. Brewer’s Spent Grains Protects against Oxidative DNA Damage in Saccharomyces cerevisiae. Journal of Agricultural Science 9, 12-21 |
Resumo(s): | Brewer’s spent grain (BSG), obtained from barley malt during brewing, contains high amounts of phenolic acids, predominantly ferulic and p-coumaric acids. The protective effects of BSG extracts against oxidative DNA damage induced by H2O2 in Saccharomyces cerevisiae cells were investigated using an optimized yeast comet assay and flow cytometry. The results indicated that BSG extracts from black malt exhibited a 5-fold reduction in the genotoxic effects of H2O2, compared to the 2-fold decrease by the BSG extracts from pilsen malts. Flow cytometry analysis with dichlorofluorescein diacetate demonstrated that the intracellular oxidation of S. cerevisiae is also reduced to approximately 50% in the presence of 20-fold diluted BSG extracts. BSG extracts obtained from pilsen and black malt types exert dose-dependent protective properties against the genotoxic effects induced by ROS and decrease intracellular oxidation of yeast cells. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/50152 |
DOI: | 10.5539/jas.v9n7p12 |
ISSN: | 1916-9752 |
e-ISSN: | 1916-9760 |
Versão da editora: | https://doi.org/10.5539/jas.v9n7p12 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Moreira et al 2017.pdf | 380,41 kB | Adobe PDF | Ver/Abrir |