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https://hdl.handle.net/1822/50276
Título: | Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients |
Autor(es): | Gonçalves, Samuel Martins Lagrou, Katrien Rodrigues, Claudia S. Campos, Claudia F. Bernal-Martinez, Leticia Rodrigues, Fernando José dos Santos Silvestre, Ricardo Jorge Leal Alcazar-Fuoli, Laura Maertens, Johan A. Cunha, Cristina Carvalho, Agostinho |
Palavras-chave: | Invasive pulmonary aspergillosis Biomarkers Cytokines/chemokines Bronchoalveolar lavage Fungal diagnostics |
Data: | 29-Nov-2017 |
Editora: | Frontiers Media |
Revista: | Frontiers in Microbiology |
Citação: | Gonçalves, S. M., Lagrou, K., Rodrigues, C. S., Campos, C. F., Bernal-Martínez, L., Rodrigues, F., ... & Carvalho, A. (2017). Evaluation of bronchoalveolar lavage fluid cytokines as biomarkers for invasive pulmonary aspergillosis in at-risk patients. Frontiers in microbiology, 8, 2362. |
Resumo(s): | Background: Invasive pulmonary aspergillosis (IPA) is an infection that primarily affects immunocompromised hosts, including hematological patients and stem-cell transplant recipients. The diagnosis of IPA remains challenging, making desirable the availability of new specific biomarkers. High-throughput methods now allow us to interrogate the immune system for multiple markers of inflammation with enhanced resolution.Methods: To determine whether a signature of alveolar cytokines could be associated with the development of IPA and used as a diagnostic biomarker, we performed a nested case-control study involving 113 patients at-risk.Results: Among the 32 analytes tested, IL-beta, IL-6, IL-8, IL-17A, IL-23, and TNF a were significantly increased among patients with IPA, defining two clusters able to accurately differentiate cases of infection from controls. Genetic variants previously reported to confer increased risk of IPA compromised the production of specific cytokines and impaired their discriminatory potential toward infection. Collectively, our data indicated that IL-8 was the best performing cytokine, with alveolar levels >= 904 pg/mL predicting IPA with elevated sensitivity (90%), specificity (73%), and negative predictive value (88%).Conclusions: These findings highlight the existence of a specific profile of alveolar cytokines, with IL-8 being the dominant discriminator, whichmight be useful in supporting current diagnostic approaches for IPA. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/50276 |
DOI: | 10.3389/fmicb.2017.02362 |
ISSN: | 1664-302X |
Versão da editora: | https://www.frontiersin.org/articles/10.3389/fmicb.2017.02362/full |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2017.Gonçalves SM.Evaluation of bronchoalveolar lavage fluid cytokines as biomarkers for invasive pulmonary aspergillosis in at-risk patients.pdf | 4,37 MB | Adobe PDF | Ver/Abrir |