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TitleOrganic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows
Author(s)Palakawong Na Ayudthaya, Susakul
van de Weijer, Antonius H. P.
van Gelder, Antonie H.
Stams, Alfons Johannes Maria
de Vos, Willem M.
Plugge, Caroline M.
KeywordsLactate fermentation
Microbial communities
Renewable energy
Rumen fluid
Organic acids
Starch waste
Issue date25-Jan-2018
PublisherSpringer Nature
JournalBiotechnology for Biofuels
CitationPalakawong Na Ayudthaya, Susakul; van de Weijer, Antonius H. P.; van Gelder, Antonie H.; Stams, A. J. M.; de Vos, Willem M.; Plugge, Caroline M., Organic acid production from potato starch waste fermentation by rumen microbial communities from Dutch and Thai dairy cows. Biotechnology for Biofuels, 11(13), 2018
Abstract(s)Background: Exploring different microbial sources for biotechnological production of organic acids is important. Dutch and Thai cow rumen samples were used as inocula to produce organic acid from starch waste in anaerobic reactors. Organic acid production profiles were determined and microbial communities were compared using 16S ribosomal ribonucleic acid gene amplicon pyrosequencing. Results In both reactors, lactate was the main initial product and was associated with growth of Streptococcus spp. (86% average relative abundance). Subsequently, lactate served as a substrate for secondary fermentations. In the reactor inoculated with rumen fluid from the Dutch cow, the relative abundance of Bacillus and Streptococcus increased from the start, and lactate, acetate, formate and ethanol were produced. From day 1.33 to 2, lactate and acetate were degraded, resulting in butyrate production. Butyrate production coincided with a decrease in relative abundance of Streptococcus spp. and increased relative abundances of bacteria of other groups, including Parabacteroides, Sporanaerobacter, Helicobacteraceae, Peptostreptococcaceae and Porphyromonadaceae. In the reactor with the Thai cow inoculum, Streptococcus spp. also increased from the start. When lactate was consumed, acetate, propionate and butyrate were produced (day 34). After day 3, bacteria belonging to five dominant groups, Bacteroides, Pseudoramibacter_Eubacterium, Dysgonomonas, Enterobacteriaceae and Porphyromonadaceae, were detected and these showed significant positive correlations with acetate, propionate and butyrate levels. Conclusions The complexity of rumen microorganisms with high adaptation capacity makes rumen fluid a suitable source to convert organic waste into valuable products without the addition of hydrolytic enzymes. Starch waste is a source for organic acid production, especially lactate.
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AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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