Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/51655

Registo completo
Campo DCValorIdioma
dc.contributor.authorCastro, Pedro Humbertopor
dc.contributor.authorCouto, Danielpor
dc.contributor.authorFreitas, Sarapor
dc.contributor.authorVerde, Nunopor
dc.contributor.authorMacho, Alberto P.por
dc.contributor.authorHuguet, Stéphaniepor
dc.contributor.authorBotella, Miguel Angelpor
dc.contributor.authorRuiz-Albert, Javierpor
dc.contributor.authorTavares, R. M.por
dc.contributor.authorBejarano, Eduardo Rodríguezpor
dc.contributor.authorAzevedo, Herlânder Anselmo Queirós Pereirapor
dc.date.accessioned2018-03-07T09:22:19Z-
dc.date.issued2016-09-
dc.identifier.issn0167-4412por
dc.identifier.urihttps://hdl.handle.net/1822/51655-
dc.description.abstractSumoylation is an essential post-translational regulator of plant development and the response to environmental stimuli. SUMO conjugation occurs via an E1-E2-E3 cascade, and can be removed by SUMO proteases (ULPs). ULPs are numerous and likely to function as sources of specificity within the pathway, yet most ULPs remain functionally unresolved. In this report we used loss-of-function reverse genetics and transcriptomics to functionally characterize Arabidopsis thaliana ULP1c and ULP1d SUMO proteases. GUS reporter assays implicated ULP1c/d in various developmental stages, and subsequent defects in growth and germination were uncovered using loss-of-function mutants. Microarray analysis evidenced not only a deregulation of genes involved in development, but also in genes controlled by various drought-associated transcriptional regulators. We demonstrated that ulp1c ulp1d displayed diminished in vitro root growth under low water potential and higher stomatal aperture, yet leaf transpirational water loss and whole drought tolerance were not significantly altered. Generation of a triple siz1 ulp1c ulp1d mutant suggests that ULP1c/d and the SUMO E3 ligase SIZ1 may display separate functions in development yet operate epistatically in response to water deficit. We provide experimental evidence that Arabidopsis ULP1c and ULP1d proteases act redundantly as positive regulators of growth, and operate mainly as isopeptidases downstream of SIZ1 in the control of water deficit responses.por
dc.description.sponsorshipThis work was supported by FEDER through the Operational Competitiveness Program-COMPETE-and by national funds through the Foundation for Science and Technology-FCT-within the scope of project "SUMOdulator" [Refs. FCOMP-01-0124-FEDER-028459 and PTDC/BIA-PLA/3850/2012]. PHC was supported by FCT [grant ref. SFRH/BD/44484/2008 and PTDC/BIA-PLA/3850/2012]. HA was supported by the "Genomics and Evolutionary Biology" project, co-financed by North Portugal Regional Operational Programme 2007/2013 (ON.2-O Novo Norte), under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF), and by FEDER (COMPETE) and FCT, for Rede de Investigacao em Biodiversidade e Biologia Evolutiva [UID/BIA/50027/2013 and POCI-01-0145-FEDER-006821].por
dc.language.isoengpor
dc.publisherSpringerpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/130409/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/COMPETE/130409/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F44484%2F2008/PTpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147365/PTpor
dc.rightsclosedAccesspor
dc.subjectAbscisic Acidpor
dc.subjectArabidopsispor
dc.subjectArabidopsis Proteinspor
dc.subjectGene Expression Regulation, Plantpor
dc.subjectGerminationpor
dc.subjectOsmoregulationpor
dc.subjectABApor
dc.subjectOsmotic stresspor
dc.subjectPlant developmentpor
dc.subjectStomatal aperturepor
dc.subjectSIZ1por
dc.subjectSUMO proteasespor
dc.titleSUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thalianapor
dc.typearticlepor
dc.peerreviewedyespor
oaire.citationStartPage143-59por
oaire.citationEndPage159por
oaire.citationIssue1-2por
oaire.citationVolume92por
dc.identifier.doi10.1007/s11103-016-0500-9por
dc.identifier.pmid27325215por
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
dc.subject.wosScience & Technologypor
sdum.journalPlant Molecular Biologypor
Aparece nas coleções:CBFP - Artigos/Papers
DBio - Artigos/Papers

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
sumo.pdf
Acesso restrito!
6,81 MBAdobe PDFVer/Abrir

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID